Application
Fig1: Western blot analysis of CAMK4 on human CAMK4 recombinant protein using anti-CAMK4 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of CAMK4 on HEK293 (1) and CAMK4-hIgGFc transfected HEK293 (2) cell lysate using anti-CAMK4 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of CAMK4 on different cell lysate using anti-CAMK4 antibody at 1/1,000dilution.Positive control：Line1:JurkatLine2:SK-N-SHLine3:RajiLine4:HeLaApplication
Fig4: ICC staining CAMK4 (green) and Actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-CAMK4 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-CAMK4 antibody. Counter stained with hematoxylin.| Product Name | CAMK4 [B8-C5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody Brain Ca(2+) calmodulin dependent protein kinase type IV antibody Brain Ca++-calmodulin dependent protein kinase type IV antibody Calcium / calmodulin dependent protein kinase type 4 catalytic chain antibody Calcium / calmodulin dependent protein kinase type IV catalytic chain antibody Calcium/calmodulin dependent protein kinase IV antibody Calcium/calmodulin dependent protein kinase type IV antibody Calcium/calmodulin-dependent protein kinase type IV antibody CAM kinase 4 antibody CAM kinase GR antibody CAM kinase IV antibody CAM kinase-GR antibody CaMK 4 antibody CAMK GR antibody CaMK IV antibody Camk4 antibody CaMKGR antibody IV antibody KCC4_HUMAN antibody MGC36771 antibody |
| Product description | The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes designated α, β, γ and δ, which may or may not be co-expressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory Threonine residue at position 177. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody Brain Ca(2+) calmodulin dependent protein kinase type IV antibody Brain Ca++-calmodulin dependent protein kinase type IV antibody Calcium / calmodulin dependent protein kinase type 4 catalytic chain antibody Calcium / calmodulin dependent protein kinase type IV catalytic chain antibody Calcium/calmodulin dependent protein kinase IV antibody Calcium/calmodulin dependent protein kinase type IV antibody Calcium/calmodulin-dependent protein kinase type IV antibody CAM kinase 4 antibody CAM kinase GR antibody CAM kinase IV antibody CAM kinase-GR antibody CaMK 4 antibody CAMK GR antibody CaMK IV antibody Camk4 antibody CaMKGR antibody IV antibody KCC4_HUMAN antibody MGC36771 antibody |
|---|---|
| Molecular Weight(MW) | 52 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
| SwissProt ID | Q16566 |
|---|
Application
Fig1: Western blot analysis of CAMK4 on human CAMK4 recombinant protein using anti-CAMK4 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CAMK4 on HEK293 (1) and CAMK4-hIgGFc transfected HEK293 (2) cell lysate using anti-CAMK4 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of CAMK4 on different cell lysate using anti-CAMK4 antibody at 1/1,000dilution.Positive control：Line1:JurkatLine2:SK-N-SHLine3:RajiLine4:HeLa
Application
Fig4: ICC staining CAMK4 (green) and Actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-CAMK4 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-CAMK4 antibody. Counter stained with hematoxylin.| Positive Control | Human CAMK4 recombinant protein, CAMK4-hIgGFc transfected HEK293 cell lysate, Jurkat, SK-N-SH, Raji, HeLa cell lysate, HepG2, human ovarian cancer tissue, human rectum cancer tissue. |
|---|---|
| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
