Application
Fig1: Western blot analysis of MARK3 on human MARK3 recombinant protein using anti-MARK3 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of MARK3 on different cell lysate using anti-MARK3 antibody at 1/1,000 dilution. Positive control：Line1:HeLaLine2:SK-N-SHLine3:K562Line4:HCT116Line5:HEK293Line6:3T3L1Line7:NIH3T3Line8:JurkatLine9:A431Application
Fig3: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-MARK3 antibody. Counter stained with hematoxylin.Application
Fig4: Flow cytometric analysis of SK-N-SH cells with MARK3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | MARK3 [H12-G2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C-TAK1 antibody Cdc25C associated protein kinase 1 antibody Cdc25C-associated protein kinase 1 antibody cTAK1 antibody ELKL motif kinase 2 antibody EMK-2 antibody Emk2 antibody ETK 1 antibody KIAA4230 antibody KP78 antibody MAP microtubule affinity regulating kinase 3 antibody MAP/microtubule affinity-regulating kinase 3 antibody Mark3 antibody MARK3_HUMAN antibody Par 1a antibody PAR1A antibody Protein kinase STK10 antibody Ser/Thr protein kinase PAR-1 antibody Serine threonine protein kinase p78 antibody Serine/threonine-protein kinase p78 antibody SerThr protein kinase PAR 1 antibody |
| Product description | MARK3 (MAP/microtubule affinity-regulating kinase 3), also known as C-TAK1 (Cdc25C-associated protein kinase 1), EMK-2 (ELKL motif kinase 2), protein kinase STK10 or serine/threonine-protein kinase p78, is a 753 amino acid peripheral membrane protein that phosphorylates Cdc25C on Serine 216 and is ubiquitously expressed in various human tissue and cell lines. Existing as six alternatively spliced isoforms, MARK3 belongs to the Ser/Thr protein kinase family and the protein kinase superfamily. MARK3 has been suggested to mediate the binding of the 14-3-3 proteins through its kinase activity and acts as a negative regulator of mitosis. The gene encoding MARK3 maps to human chromosome 14q32.3 and mouse chromosome 12 F1. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:250~1:1000 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | C-TAK1 antibody Cdc25C associated protein kinase 1 antibody Cdc25C-associated protein kinase 1 antibody cTAK1 antibody ELKL motif kinase 2 antibody EMK-2 antibody Emk2 antibody ETK 1 antibody KIAA4230 antibody KP78 antibody MAP microtubule affinity regulating kinase 3 antibody MAP/microtubule affinity-regulating kinase 3 antibody Mark3 antibody MARK3_HUMAN antibody Par 1a antibody PAR1A antibody Protein kinase STK10 antibody Ser/Thr protein kinase PAR-1 antibody Serine threonine protein kinase p78 antibody Serine/threonine-protein kinase p78 antibody SerThr protein kinase PAR 1 antibody |
|---|---|
| Molecular Weight(MW) | 84 kDa |
| Cellular Localization | Cell membrane. Peripheral membrane protein. |
| SwissProt ID | P27448 |
|---|
Application
Fig1: Western blot analysis of MARK3 on human MARK3 recombinant protein using anti-MARK3 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of MARK3 on different cell lysate using anti-MARK3 antibody at 1/1,000 dilution. Positive control：Line1:HeLaLine2:SK-N-SHLine3:K562Line4:HCT116Line5:HEK293Line6:3T3L1Line7:NIH3T3Line8:JurkatLine9:A431
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-MARK3 antibody. Counter stained with hematoxylin.
Application
Fig4: Flow cytometric analysis of SK-N-SH cells with MARK3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human MARK3 recombinant protein, HeLa, SK-N-SH, K562, HCT116, HEK293, 3T3L1, NIH3T3, Jurkat, and A431 cell lysate, human bladder cancer tissue, SK-N-SH. |
|---|---|
| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
