Application
Fig1: Western blot analysis of ABCB5 on human ABCB5 recombinant protein using anti-ABCB5 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of ABCB5 on A431 cell lysate using anti-ABCB5 antibody at 1/1,000 dilution.Application
Fig3: ICC staining ABCB5 (green) and Actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ABCB5 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-ABCB5 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of HepG2 cells with ABCB5 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Product Name | ABCB5 [C6-H3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ABCB 5 antibody ABCB5 antibody ABCB5 P-gp antibody ABCB5_HUMAN antibody ABCB5alpha antibody ABCB5beta antibody ATP binding cassette protein antibody ATP binding cassette sub family B (MDR/TAP) member 5 antibody ATP binding cassette sub family B member 5 antibody ATP-binding cassette sub-family B member 5 antibody EST422562 antibody P glycoprotein ABCB5 antibody P-glycoprotein ABCB5 antibody |
| Product description | ATP-binding cassette (ABC) transporters are an evolutionarily conserved family of proteins that catalyze the transport of molecules across extracellular and intracellular membranes by harnessing the energy of ATP hydrolysis. ABCB5 (ATP-binding cassette sub-family B member 5), also known as P-glycoprotein ABCB5 or ABCB5 P-gp, is a 812 amino acid multi-pass membrane protein that belongs to the superfamily of ABC transporters. Expressed ubiquitously, ABCB5 contains two ABC transporter domains and one ABC transmembrane type-1 domain and is responsible for the resistance to doxorubicin of a subset of malignant melanomas. It is suggested ABCB5 inhibits tumor growth and is thought to be a novel drug transporter and chemoresistance mediator in melanoma cells. Two isoforms of ABCB5, designated alpha and beta, exist due to alternative splicing events. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | ABCB 5 antibody ABCB5 antibody ABCB5 P-gp antibody ABCB5_HUMAN antibody ABCB5alpha antibody ABCB5beta antibody ATP binding cassette protein antibody ATP binding cassette sub family B (MDR/TAP) member 5 antibody ATP binding cassette sub family B member 5 antibody ATP-binding cassette sub-family B member 5 antibody EST422562 antibody P glycoprotein ABCB5 antibody P-glycoprotein ABCB5 antibody |
|---|---|
| Molecular Weight(MW) | 90 kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | Q2M3G0 |
|---|
Application
Fig1: Western blot analysis of ABCB5 on human ABCB5 recombinant protein using anti-ABCB5 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of ABCB5 on A431 cell lysate using anti-ABCB5 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining ABCB5 (green) and Actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ABCB5 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-ABCB5 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of HepG2 cells with ABCB5 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Positive Control | Human ABCB5 recombinant protein, A431 cell lysate, human liver cancer tissue, human breast cancer tissue, HepG2. |
|---|---|
| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
