Application
Fig1: Western blot analysis of Brachyury on human Brachyury recombinant protein using anti- Brachyury antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of Brachyury on HEK293 (1) and Brachyury -hIgGFc transfected HEK293 (2) cell lysate using anti- Brachyury antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of Brachyury on Raji (1) and Jurkat (2) cell lysate using anti- Brachyury antibody at 1/1,000 dilution.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti- Brachyury antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti- Brachyury antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Hela cells with Brachyury antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | T [A2-H9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The T-box gene family consists of members that share a unique DNA binding domain. The best characterized T-box (Tbx) gene, Brachyury or T, encodes a transcription factor that plays an important role in early vertebrate development. Tbx genes are a family of developmental regulators with more than 20 members recently identified among invertebrates and vertebrates. Mutations in Tbx genes have been found to cause several human diseases. The understanding of functional mechanisms of Tbx products has come mainly from the prototypical T/Brachyury protein, which is a transcription activator. The T-domain is a highly conserved DNA-binding motif originally defined in Brachyury and characteristic of the Tbx family of transcription factors. The murine Brachyury (T) gene is required in posterior mesoderm formation and axial development. Mutant embryos lacking T gene function are deficient in notochord differentiation and posterior mesoderm formation, but develop anterior mesoderm. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:250~1:1000 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Molecular Weight(MW) | 47 kDa |
|---|---|
| Cellular Localization | Nucleus. |
| SwissProt ID | O15178 |
|---|
Application
Fig1: Western blot analysis of Brachyury on human Brachyury recombinant protein using anti- Brachyury antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of Brachyury on HEK293 (1) and Brachyury -hIgGFc transfected HEK293 (2) cell lysate using anti- Brachyury antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of Brachyury on Raji (1) and Jurkat (2) cell lysate using anti- Brachyury antibody at 1/1,000 dilution.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti- Brachyury antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti- Brachyury antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with Brachyury antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human Brachyury recombinant protein, Brachyury -hIgGFc transfected HEK293 cell lysate, Raji, Jurkat cell lysate, human cervical cancer tissue, human rectum cancer tissue, Hela. |
|---|---|
| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
