Application
Fig1: Western blot analysis of CLGN on human CLGN recombinant protein using anti-CLGN antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of CLGN on HEK293 (1) and CLGN-hIgGFc transfected HEK293 (2) cell lysate using anti-CLGN antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of CLGN on different cell lysate using anti-CLGN antibody at 1/1,000 dilution.Positive control：Line1:LNCaPLine2:HepG2Line3:PC-3Line4:RajiApplication
Fig4: ICC staining CLGN (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-CLGN antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-CLGN antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of HEK293 cells with CLGN antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Product Name | CLGN [G10-C8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 27 kDa interstitial collagenase antibody CLG antibody CLGN antibody collagenase, fibroblast antibody collagenase, interstitial antibody Fibroblast collagenase antibody Interstitial collagenase antibody Matrix metallopeptidase 1 (interstitial collagenase) antibody Matrix metalloprotease 1 antibody Matrix Metalloproteinase 1 antibody Matrix metalloproteinase-1 antibody MMP 1 antibody MMP-1 antibody MMP1 antibody MMP1_HUMAN antibody OTTHUMP00000045866 antibody |
| Product description | Functions during spermatogenesis as a chaperone for a range of client proteins that are important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. Required for normal sperm migration from the uterus into the oviduct. Required for normal male fertility. Binds calcium ions. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | 27 kDa interstitial collagenase antibody
CLG antibody
CLGN antibody
collagenase fibroblast antibody collagenase interstitial antibody Fibroblast collagenase antibody Interstitial collagenase antibody Matrix metallopeptidase 1 (interstitial collagenase) antibody Matrix metalloprotease 1 antibody Matrix Metalloproteinase 1 antibody Matrix metalloproteinase-1 antibody MMP 1 antibody MMP-1 antibody MMP1 antibody MMP1_HUMAN antibody OTTHUMP00000045866 antibody |
|---|---|
| Molecular Weight(MW) | 70 kDa |
| Cellular Localization | Endoplasmic reticulum membrane. |
| SwissProt ID | O14967 |
|---|
Application
Fig1: Western blot analysis of CLGN on human CLGN recombinant protein using anti-CLGN antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CLGN on HEK293 (1) and CLGN-hIgGFc transfected HEK293 (2) cell lysate using anti-CLGN antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of CLGN on different cell lysate using anti-CLGN antibody at 1/1,000 dilution.Positive control：Line1:LNCaPLine2:HepG2Line3:PC-3Line4:Raji
Application
Fig4: ICC staining CLGN (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-CLGN antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-CLGN antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of HEK293 cells with CLGN antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Positive Control | Human CLGN recombinant protein, CLGN-hIgGFc transfected HEK293 cell lysate, LNCaP, HepG2, PC-3, Raji cell lysate, HepG2, human breast cancer tissue, human esophageal cancer tissue, HEK293. |
|---|---|
| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
