Application
Fig1: Western blot analysis of PAX3 on human PAX3 recombinant protein using anti-PAX3 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of PAX3 on HEK293 (1) and PAX3-hIgGFc transfected HEK293 (2) cell lysate using anti-PAX3 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of PAX3 on different cell lysate using anti-PAX3 antibody at 1/1,000 dilution. Positive control：Line1:Mouse brainLine2:Rat spleenLine3:Mouse liver| Product Name | PAX3 [G7-D8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDHS antibody HUP 2 antibody HUP2 antibody MGC120381 antibody MGC120382 antibody MGC120383 antibody MGC120384 antibody MGC134778 antibody Paired box 3 antibody Paired box gene 3 antibody Paired box homeotic gene 3 antibody Paired box protein Pax 3 antibody Paired box protein Pax-3 antibody Paired box protein Pax3 antibody Paired domain gene 3 antibody Paired domain gene HuP2 antibody PAX 3 antibody Pax3 antibody PAX3/FKHR fusion gene antibody PAX3_HUMAN antibody Sp antibody splotch antibody Waardenburg syndrome 1 antibody WS 1 antibody WS1 antibody WS3 antibody |
| Product description | Pax genes contain paired domains that share strong homology to genes in Drosophila which are involved in programming early development. The product of the Pax-3 gene is a DNA-binding protein expressed during early neurogenesis. Pax-3 is a protein containing both a paired domain and a paired-type homeodomain. During early neurogenesis, Pax-3 expression is limited to mitotic cells in the ventricular zone of the developing spinal cord and to distinct regions in the hindbrain, midbrain and diencephalon. In 10-12 day embryos, expression of Pax-3 is also seen in neural crest cells of the developing spinal ganglia, the craniofacial mesectoderm and in limb mesenchyme. Mutations in the MITF and Pax-3 genes, encoding transcriptions factors, are responsible for Waardenburg syndrome II (WS2) and WS1/WS3, respectively. Pax-7 is a gene specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization revealed that Pax-7 is also expressed in satellite cells residing in adult muscle. The gene which encodes Pax-7 maps to human chromosome 1p36.2-p36.12. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500-1:1,000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | CDHS antibody HUP 2 antibody HUP2 antibody MGC120381 antibody MGC120382 antibody MGC120383 antibody MGC120384 antibody MGC134778 antibody Paired box 3 antibody Paired box gene 3 antibody Paired box homeotic gene 3 antibody Paired box protein Pax 3 antibody Paired box protein Pax-3 antibody Paired box protein Pax3 antibody Paired domain gene 3 antibody Paired domain gene HuP2 antibody PAX 3 antibody Pax3 antibody PAX3/FKHR fusion gene antibody PAX3_HUMAN antibody Sp antibody splotch antibody Waardenburg syndrome 1 antibody WS 1 antibody WS1 antibody WS3 antibody |
|---|---|
| Molecular Weight(MW) | 53 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of PAX3 on human PAX3 recombinant protein using anti-PAX3 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PAX3 on HEK293 (1) and PAX3-hIgGFc transfected HEK293 (2) cell lysate using anti-PAX3 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of PAX3 on different cell lysate using anti-PAX3 antibody at 1/1,000 dilution. Positive control：Line1:Mouse brainLine2:Rat spleenLine3:Mouse liver| Positive Control | Human PAX3 recombinant protein, PAX3-hIgGFc transfected HEK293 cell lysate, Mouse brain, Rat spleen, Mouse liver cell lysate. |
|---|---|
| Application Notes | WB:1:500-1:1,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
