Application
Fig1: Western blot analysis of IL3RA on human IL3RA recombinant protein using anti-IL3RA antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of IL3RA on HEK293 (1) and IL3RA-hIgGFc transfected HEK293 (2) cell lysate using anti-IL3RA antibody at 1/1,000 dilution.Application
Fig3: ICC staining IL3RA (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-IL3RA antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-IL3RA antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Hela cells with IL3RA antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | IL3RA [C5-E11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD123 antibody CD123 antigen antibody hIL 3Ra antibody hIL-3Ra antibody hIL3Ra antibody IL 3 receptor alpha SP2 isoform antibody IL 3R alpha antibody IL-3 receptor subunit alpha antibody IL-3R subunit alpha antibody IL-3R-alpha antibody IL-3RA antibody IL3 Receptor alpha antibody IL3R antibody Il3ra antibody IL3RA_HUMAN antibody IL3RAX antibody IL3RAY antibody IL3RX antibody IL3RY antibody Interleukin 3 receptor alpha chain antibody Interleukin 3 receptor, alpha (low affinity) antibody Interleukin 3 receptor, alpha antibody Interleukin-3 Receptor alpha antibody Interleukin-3 receptor subunit alpha antibody Interleukin3 receptor antibody Interleukin3 receptor, Y-chromosomal antibody |
| Product description | Interleukin-3, or IL-3, is a pleiotropic cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL-3 exerts its biological effects through a receptor which consists of a ligand-specific α subunit (IL-3Rα) and a signal transducing β subunit (IL-3Rβ) common to the IL-3/IL-5/GM-CSF receptors. The α subunits are low-affinity ligand-binding proteins while the β subunits do not themselves bind ligand, but are required for high affinity binding by the α subunits. The mouse IL-3 receptor has two distinct β subunits, one that functions only in IL-3-mediated cell signaling and a second that is shared with IL-5 and GM-CSF. The murine β subunits are 91% homologous at the amino acid level but only 56% homologous to the human β subunit. The carboxy-terminus of the β subunit has been shown to be necessary for activation of the MAP kinase signaling pathway. Although the IL-3 receptor has no intrinsic kinase activity, stimulation with IL-3 leads to tyrosine phosphorylation of the JAK/Tyk 2 family member, JAK2, which in turn activates and causes nuclear translocation of Stat5a and Stat5b. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | CD123 antibody
CD123 antigen antibody
hIL 3Ra antibody
hIL-3Ra antibody
hIL3Ra antibody
IL 3 receptor alpha SP2 isoform antibody
IL 3R alpha antibody
IL-3 receptor subunit alpha antibody
IL-3R subunit alpha antibody
IL-3R-alpha antibody
IL-3RA antibody
IL3 Receptor alpha antibody
IL3R antibody
Il3ra antibody
IL3RA_HUMAN antibody
IL3RAX antibody
IL3RAY antibody
IL3RX antibody
IL3RY antibody
Interleukin 3 receptor alpha chain antibody
Interleukin 3 receptor alpha (low affinity) antibody Interleukin 3 receptor alpha antibody Interleukin-3 Receptor alpha antibody Interleukin-3 receptor subunit alpha antibody Interleukin3 receptor antibody Interleukin3 receptor Y-chromosomal antibody |
|---|---|
| Molecular Weight(MW) | 43 kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | P26951 |
|---|
Application
Fig1: Western blot analysis of IL3RA on human IL3RA recombinant protein using anti-IL3RA antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of IL3RA on HEK293 (1) and IL3RA-hIgGFc transfected HEK293 (2) cell lysate using anti-IL3RA antibody at 1/1,000 dilution.
Application
Fig3: ICC staining IL3RA (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-IL3RA antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-IL3RA antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with IL3RA antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human IL3RA recombinant protein, IL3RA-hIgGFc transfected HEK293 cell lysate, human ovarian cancer tissue, human lung cancer tissue, Hela. |
|---|---|
| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
