Application
Fig1: Western blot analysis of CD33 on human CD33 recombinant protein using anti-CD33 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of CD33 on HEK293 (1) and CD33-hIgGFc transfected HEK293 (2) cell lysate using anti-CD33 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of CD33 on THP-1 (1) cell lysate using anti-CD33 antibody at 1/1,000 dilution.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-CD33 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human endometrium tissue using anti-CD33 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Hela cells with CD33 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Product Name | CD33 [B7-C6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD 33 antibody CD33 antibody CD33 antigen (gp67) antibody CD33 antigen antibody CD33 molecule antibody CD33_HUMAN antibody FLJ00391 antibody gp67 antibody My9 antibody Myeloid cell surface antigen CD33 antibody Myeloid cell surface antigen CD33 precursor antibody Myeloid differentiation antigen CD33 antibody p67 antibody Sialic acid binding Ig like lectin 3 antibody Sialic acid binding immunoglobulin like lectin 3 antibody Sialic acid-binding Ig-like lectin 3 antibody SIGLEC 3 antibody Siglec-3 antibody SIGLEC3 antibody |
| Product description | CD33 is a type I transmembrane glycoprotein that is found on granulocyte and macrophage precursors in the bone marrow, but is absent from pluripotent stem cells. CD33 is also expressed on monocytes in peripheral blood. It is used as a marker to distinguish myelogenous leukemia cells from lymphoid or erythroid leukemias. CD33 may function as a sialic acid-dependent cell adhesion molecule. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:250~1:1000 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | CD 33 antibody CD33 antibody CD33 antigen (gp67) antibody CD33 antigen antibody CD33 molecule antibody CD33_HUMAN antibody FLJ00391 antibody gp67 antibody My9 antibody Myeloid cell surface antigen CD33 antibody Myeloid cell surface antigen CD33 precursor antibody Myeloid differentiation antigen CD33 antibody p67 antibody Sialic acid binding Ig like lectin 3 antibody Sialic acid binding immunoglobulin like lectin 3 antibody Sialic acid-binding Ig-like lectin 3 antibody SIGLEC 3 antibody Siglec-3 antibody SIGLEC3 antibody |
|---|---|
| Molecular Weight(MW) | 70 kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P20138 |
|---|
Application
Fig1: Western blot analysis of CD33 on human CD33 recombinant protein using anti-CD33 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CD33 on HEK293 (1) and CD33-hIgGFc transfected HEK293 (2) cell lysate using anti-CD33 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of CD33 on THP-1 (1) cell lysate using anti-CD33 antibody at 1/1,000 dilution.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-CD33 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human endometrium tissue using anti-CD33 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with CD33 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Positive Control | Human CD33 recombinant protein, CD33-hIgGFc transfected HEK293 cell lysate, THP-1 cell lysate, human esophageal cancer tissue, human endometrium tissue, Hela. |
|---|---|
| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
