Application
Fig1: Western blot analysis of GOT2 on different cell lysate using anti-GOT2 antibody at 1/1,000 dilution. Positive control：Line1: HEK293Line2: PC-12Line3: HL-60Line4: BCBL-1Line5: HepG2Line6: NIH/3T3Application
Fig2: ICC staining GOT2 (green) and Actin filaments (red) in PC-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining GOT2 (green) and Actin filaments (red) in SK-Br-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | GOT2 [D6-E9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AATM_HUMAN antibody AL022787 antibody Aspartate aminotransferase 2 antibody Aspartate aminotransferase antibody Aspartate aminotransferase, mitochondrial antibody Aspartate transaminase 2 antibody ASPATA antibody EC 2.6.1.1 antibody FABP 1 antibody FABP pm antibody FABP-1 antibody FABPpm antibody Fatty acid binding protein antibody Fatty acid-binding protein antibody FLJ40994 antibody Glutamate oxaloacetate transaminase 2 antibody Glutamate oxaloacetate transaminase 2, mitochondrial antibody Glutamate oxaloacetate transaminase, mitochondrial antibody Glutamic oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) antibody Got 2 antibody GOT2 antibody KAT4 antibody KATIV antibody kynurenine aminotransferase 4 antibody Kynurenine aminotransferase IV antibody kynurenineoxoglutarate transaminase 4 antibody kynurenineoxoglutarate transaminase IV antibody mAspAT antibody MGC102129 antibody MGC115763 antibody mitAAT antibody mitochondrial antibody Mitochondrial aspartate aminotransferase antibody OTTMUSP00000017748 antibody Plasma membrane fatty acid binding protein antibody Plasma membrane-associated fatty acid-binding protein antibody Transaminase A antibody |
| Product description | Aspartate aminotransferase (AAT) is a ubiquitous pyridoxal phosphate-dependent enzyme, which exists in both mitochondrial (AATM) and cytosolic (AATC) forms. The enzyme plays an important role in amino acid metabolism and in the urea and tricarboxylic acid cycles by catalyzing the conversion of L-aspartate and 2-oxoglutarate to oxaloacetate and L-glutamate. The two isoenzymes are homodimeric, but differ in expression patterns. Approximately 80% of the enzyme activity in liver is of mitochondrial origin, whereas in serum the enzyme activity is largely cytosolic. AATC and AATM share nearly identical three-dimensional structures, but differ in their folding rates and in their affinity for binding to molecular chaperones, including GroEL. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICC |
| WB:1:250~1:1000 ICC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRatMonkey |
| Concentration | 2mg/ml |
| Alternative Names | AATM_HUMAN antibody
AL022787 antibody
Aspartate aminotransferase 2 antibody
Aspartate aminotransferase antibody
Aspartate aminotransferase mitochondrial antibody Aspartate transaminase 2 antibody ASPATA antibody EC 2.6.1.1 antibody FABP 1 antibody FABP pm antibody FABP-1 antibody FABPpm antibody Fatty acid binding protein antibody Fatty acid-binding protein antibody FLJ40994 antibody Glutamate oxaloacetate transaminase 2 antibody Glutamate oxaloacetate transaminase 2 mitochondrial antibody Glutamate oxaloacetate transaminase mitochondrial antibody Glutamic oxaloacetic transaminase 2 mitochondrial (aspartate aminotransferase 2) antibody Got 2 antibody GOT2 antibody KAT4 antibody KATIV antibody kynurenine aminotransferase 4 antibody Kynurenine aminotransferase IV antibody kynurenineoxoglutarate transaminase 4 antibody kynurenineoxoglutarate transaminase IV antibody mAspAT antibody MGC102129 antibody MGC115763 antibody mitAAT antibody mitochondrial antibody Mitochondrial aspartate aminotransferase antibody OTTMUSP00000017748 antibody Plasma membrane fatty acid binding protein antibody Plasma membrane-associated fatty acid-binding protein antibody Transaminase A antibody |
|---|---|
| Molecular Weight(MW) | 47 kDa |
| Cellular Localization | Mitochondrion matrix. Cell membrane. |
Application
Fig1: Western blot analysis of GOT2 on different cell lysate using anti-GOT2 antibody at 1/1,000 dilution. Positive control：Line1: HEK293Line2: PC-12Line3: HL-60Line4: BCBL-1Line5: HepG2Line6: NIH/3T3
Application
Fig2: ICC staining GOT2 (green) and Actin filaments (red) in PC-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining GOT2 (green) and Actin filaments (red) in SK-Br-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | HEK293, PC-12, BCBL-1, HepG2, NIH/3T3 cell lysate, PC-3, SK-Br-3. |
|---|---|
| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
