Application
Fig1: Western blot analysis of KLHL13 on Hela(1) and MCF-7 (2) cell lysate using anti-KLHL13 antibody at 1/1,000 dilution.Application
Fig2: ICC staining KLHL13 (green) and Actin filaments (red) in NTERA-2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining KLHL13 (green) and Actin filaments (red) in U251 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-KLHL13 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-KLHL13 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of 3T3/L1 cells with KLHL13 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Product Name | KLHL13 [B12-D1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BKLHD2 antibody BTB and kelch domain containing 2 antibody BTB and kelch domain containing 2 KIAA1309 antibody BTB and kelch domain containing protein 2 antibody BTB and kelch domain-containing protein 2 antibody FLJ10262 antibody Kelch like 13 (Drosophila) antibody Kelch like protein 13 antibody Kelch-like protein 13 antibody KIAA1309 antibody KLH13_HUMAN antibody KLHL13 antibody MGC74791 antibody |
| Product description | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis. The BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex mediates the ubiquitination of AURKB and controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | BKLHD2 antibody BTB and kelch domain containing 2 antibody BTB and kelch domain containing 2 KIAA1309 antibody BTB and kelch domain containing protein 2 antibody BTB and kelch domain-containing protein 2 antibody FLJ10262 antibody Kelch like 13 (Drosophila) antibody Kelch like protein 13 antibody Kelch-like protein 13 antibody KIAA1309 antibody KLH13_HUMAN antibody KLHL13 antibody MGC74791 antibody |
|---|---|
| Molecular Weight(MW) | 74 kDa |
| Cellular Localization | Cytosol. |
| SwissProt ID | Q9P2N7 |
|---|
Application
Fig1: Western blot analysis of KLHL13 on Hela(1) and MCF-7 (2) cell lysate using anti-KLHL13 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining KLHL13 (green) and Actin filaments (red) in NTERA-2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining KLHL13 (green) and Actin filaments (red) in U251 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-KLHL13 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-KLHL13 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of 3T3/L1 cells with KLHL13 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Positive Control | Hela, MCF-7 cell lysate, human brain tissue, human pancreas tissue, NTERA-2, U251, 3T3/L1. |
|---|---|
| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
