Application
Fig1: Western blot analysis of KLHL11 on Hela and MCF-7 cell lysate using anti-KLHL11 antibody at 1/1,000 dilution.Application
Fig2: ICC staining KLHL11 (green) and Actin filaments (red) in 3T3-L1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Flow cytometric analysis of K562 cells with KLHL11 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Product Name | KLHL11 [A8-B9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ10572 antibody Kelch like 11 (Drosophila) antibody Kelch like 11 antibody Kelch like protein 11 antibody Kelch-like protein 11 antibody KLH11_HUMAN antibody KLHL 11 antibody Klhl11 antibody |
| Product description | Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICC/IFFC |
| WB:1:250~1:1000 ICC/IF:1:100~1:200 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | FLJ10572 antibody Kelch like 11 (Drosophila) antibody Kelch like 11 antibody Kelch like protein 11 antibody Kelch-like protein 11 antibody KLH11_HUMAN antibody KLHL 11 antibody Klhl11 antibody |
|---|---|
| Molecular Weight(MW) | 80 kDa |
| Cellular Localization | Cytosol, nucleus. |
| SwissProt ID | Q9NVR0 |
|---|
Application
Fig1: Western blot analysis of KLHL11 on Hela and MCF-7 cell lysate using anti-KLHL11 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining KLHL11 (green) and Actin filaments (red) in 3T3-L1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Flow cytometric analysis of K562 cells with KLHL11 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Positive Control | Hela, MCF-7 cell lysate, 3T3-L1, K562. |
|---|---|
| Application Notes | WB:1:250~1:1000 ICC/IF:1:100~1:200 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
