Application
Fig1: Western blot analysis of HAS2 on human HAS2 recombinant protein using anti-HAS2 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of HAS2 on NTERA-2 (1) and HEK293 (2) cell lysate using anti-HAS2 antibody at 1/1,000 dilution.Application
Fig3: ICC staining HAS2 (green) and Actin filaments (red) in HeLa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-HAS2 antibody. Counter stained with hematoxylin.| Product Name | HAS2 [C4-E7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HA synthase 2 antibody has2 antibody HAS2_HUMAN antibody Hyaluronan synthase 2 antibody Hyaluronate synthase 2 antibody Hyaluronic acid synthase 2 antibody |
| Product description | HAS1, HAS2 and HAS3 are HA Synthase proteins that synthesize HA (Hyaluronan or hyaluronic acid). The extracellular matrix in most vertebrates express HA, which is a high molecular weight linear polysaccharide composed of alternating glucuronic acid and N-acetylglucosamine residues linked by β-1,3 and β-1,4 glycosidic bonds. The three HAS genes show distinct patterns of expression during development and their protein products play significantly different roles in the formation of the HA matrix. Both HAS1 and HAS2 synthesise high molecular-weight HA, whereas HAS3 produces lower molecular weight HA. The expression of the three HAS isoforms is more prominent in growing cells than in resting cells and is differentially regulated by various stimuli suggesting distinct functional roles of the three proteins. HAS2 mRNA shows predominant expression in chondrocytes and cartilage. The human HAS2 gene maps to chromosome 8q24.12. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHC |
| WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | HA synthase 2 antibody has2 antibody HAS2_HUMAN antibody Hyaluronan synthase 2 antibody Hyaluronate synthase 2 antibody Hyaluronic acid synthase 2 antibody |
|---|---|
| Molecular Weight(MW) | 64 kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | Q92819 |
|---|
Application
Fig1: Western blot analysis of HAS2 on human HAS2 recombinant protein using anti-HAS2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of HAS2 on NTERA-2 (1) and HEK293 (2) cell lysate using anti-HAS2 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining HAS2 (green) and Actin filaments (red) in HeLa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-HAS2 antibody. Counter stained with hematoxylin.| Positive Control | Human HAS2 recombinant protein, NTERA-2, HEK293 cell lysate, HeLa, human ovarian cancer tissue. |
|---|---|
| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
