Application
Fig1: Western blot analysis of IL34 on IL34-hIgGFc transfected HEK293 cell lysate using anti-IL34 antibody at 1/1,000 dilution.Application
Fig2: ICC staining IL34 (green) and Actin filaments (red) in U251 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Flow cytometric analysis of K562 cells with IL34 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Product Name | IL34 [E8-D12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C16orf77 antibody Chromosome 16 open reading frame 77 antibody FPT025 antibody IL 34 antibody IL-34 antibody Il34 antibody IL34_HUMAN antibody Interleukin 34 antibody Interleukin-34 antibody Interleukin34 antibody MGC34647 antibody |
| Product description | Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICC/IFFC |
| WB:1:250~1:1000 ICC/IF:1:100~1:200 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | C16orf77 antibody Chromosome 16 open reading frame 77 antibody FPT025 antibody IL 34 antibody IL-34 antibody Il34 antibody IL34_HUMAN antibody Interleukin 34 antibody Interleukin-34 antibody Interleukin34 antibody MGC34647 antibody |
|---|---|
| Molecular Weight(MW) | 27 kDa |
| Cellular Localization | Secreted. |
| SwissProt ID | Q6ZMJ4 |
|---|
Application
Fig1: Western blot analysis of IL34 on IL34-hIgGFc transfected HEK293 cell lysate using anti-IL34 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining IL34 (green) and Actin filaments (red) in U251 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Flow cytometric analysis of K562 cells with IL34 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Positive Control | IL34-hIgGFc transfected HEK293 cell lysate, U251, K562. |
|---|---|
| Application Notes | WB:1:250~1:1000 ICC/IF:1:100~1:200 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
