Application
Fig1: Western blot analysis of DLK1 on human DLK1 recombinant protein using anti-DLK1 antibody at 1/1,000 dilution.Application
Fig2: ICC staining DLK1 (green) and actin filaments (red) in U251 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3:Flow cytometric analysis of NIH/3T3 cells with DLK1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | DLK1 [D2-A3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Adipocyte differentiation inhibitor protein antibody Brevideltin antibody Delta like 1 homolog antibody Delta like homolog antibody Delta like protein antibody Delta1 antibody DLK 1 antibody DLK antibody DLK-1 antibody DLK1 antibody DLK1_HUMAN antibody FA 1 antibody FA1 antibody Fetal antigen 1 antibody pG2 antibody Preadipocyte factor 1 antibody Pref 1 antibody Pref antibody Pref1 antibody Protein delta homolog 1 antibody Secredeltin antibody ZOG antibody |
| Product description | The DLK1 protein containing six epidermal growth factor repeats. The protein is involved in the differentiation of several cell types, including adipocytes; it is also thought to be a tumor suppressor. It is one of several imprinted genes located in a region of on chr 14q32. Certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). This proteins' gene is expressed from the paternal allele. A polymorphism within this gene has been associated with child and adolescent obesity. The mode of inheritance for this polymorphism is polar overdominance; this non-Mendelian inheritance pattern was first described in sheep with the callipyge phenotype, which is characterized by muscle hypertrophy and decreased fat mass. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | Adipocyte differentiation inhibitor protein antibody Brevideltin antibody Delta like 1 homolog antibody Delta like homolog antibody Delta like protein antibody Delta1 antibody DLK 1 antibody DLK antibody DLK-1 antibody DLK1 antibody DLK1_HUMAN antibody FA 1 antibody FA1 antibody Fetal antigen 1 antibody pG2 antibody Preadipocyte factor 1 antibody Pref 1 antibody Pref antibody Pref1 antibody Protein delta homolog 1 antibody Secredeltin antibody ZOG antibody |
|---|---|
| Molecular Weight(MW) | 41 kDa |
| Cellular Localization | Membrane. |
Application
Fig1: Western blot analysis of DLK1 on human DLK1 recombinant protein using anti-DLK1 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining DLK1 (green) and actin filaments (red) in U251 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3:Flow cytometric analysis of NIH/3T3 cells with DLK1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human DLK1 recombinant protein, U251, NIH/3T3. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
