Application
Fig1: Western blot analysis of RSK2 on human RSK2 recombinant protein using anti-RSK2 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of RSK2 on different cell lysates using anti-RSK2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: HepG2Application
Fig3: ICC staining RSK2 (green) and actin filaments (red) in epG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4:Flow cytometric analysis of HepG2 cells with RSK2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | RSK2 [D2-E10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 90 kDa ribosomal protein S6 kinase 3 antibody CLS antibody HU 3 antibody HU2 antibody HU3 antibody Insulin stimulated protein kinase 1 antibody Insulin-stimulated protein kinase 1 antibody ISPK-1 antibody ISPK1 antibody KS6A3_HUMAN antibody MAP kinase activated protein kinase 1b antibody MAP kinase-activated protein kinase 1b antibody MAPK activated protein kinase 1b antibody MAPK-activated protein kinase 1b antibody MAPKAP kinase 1b antibody MAPKAPK 1b antibody MAPKAPK-1b antibody MAPKAPK1B antibody Mental retardation, X linked 19 antibody MRX19 antibody OTTHUMP00000023036 antibody p90 RSK2 antibody p90 RSK3 antibody p90-RSK 3 antibody p90RSK3 antibody pp90RSK2 antibody Ribosomal protein S6 kinase 90kDa polypeptide 3 antibody Ribosomal protein S6 kinase alpha 3 antibody Ribosomal protein S6 kinase alpha-3 antibody Ribosomal protein s6 kinase ii alpha 2 antibody Ribosomal S6 kinase 2 antibody Rps6ka3 antibody RSK antibody RSK-2 antibody RSK2 antibody S6 kinase 2 antibody S6K alpha3 antibody S6K-alpha-3 antibody |
| Product description | The family of ribosomal S6 kinases (Rsks), designated Rsk-1 (or MAPKAP kinase-1), Rsk-2 and Rsk-3, are intracellular serine/threonine kinases that are important signaling intermediates in response to a broad range of ligand activated receptor tyrosine kinases. A unique feature common to the members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. An additional Rsk protein, Rsk-4, shows a high level of homology to the three previously isolated members of the human Rsk family. Rsk-4 is most abundantly expressed in brain and kidney and plays a role in normal neuronal development. The family of ribosomal S6 kinases includes p70 S6 kinase and p70 S6 kinase b, which are thought to have similar regulatory functions. MSK1 (also designated RLPK) is a novel Rsk-related protein, which, like the p90 Rsk family members, contains two non-identical complete kinase catalytic domains. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | 90 kDa ribosomal protein S6 kinase 3 antibody
CLS antibody
HU 3 antibody
HU2 antibody
HU3 antibody
Insulin stimulated protein kinase 1 antibody
Insulin-stimulated protein kinase 1 antibody
ISPK-1 antibody
ISPK1 antibody
KS6A3_HUMAN antibody
MAP kinase activated protein kinase 1b antibody
MAP kinase-activated protein kinase 1b antibody
MAPK activated protein kinase 1b antibody
MAPK-activated protein kinase 1b antibody
MAPKAP kinase 1b antibody
MAPKAPK 1b antibody
MAPKAPK-1b antibody
MAPKAPK1B antibody
Mental retardation X linked 19 antibody MRX19 antibody OTTHUMP00000023036 antibody p90 RSK2 antibody p90 RSK3 antibody p90-RSK 3 antibody p90RSK3 antibody pp90RSK2 antibody Ribosomal protein S6 kinase 90kDa polypeptide 3 antibody Ribosomal protein S6 kinase alpha 3 antibody Ribosomal protein S6 kinase alpha-3 antibody Ribosomal protein s6 kinase ii alpha 2 antibody Ribosomal S6 kinase 2 antibody Rps6ka3 antibody RSK antibody RSK-2 antibody RSK2 antibody S6 kinase 2 antibody S6K alpha3 antibody S6K-alpha-3 antibody |
|---|---|
| Molecular Weight(MW) | 84 kDa |
| Cellular Localization | Nucleus, Cytoplasm. |
| SwissProt ID | P51812 |
|---|
Application
Fig1: Western blot analysis of RSK2 on human RSK2 recombinant protein using anti-RSK2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of RSK2 on different cell lysates using anti-RSK2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: HepG2
Application
Fig3: ICC staining RSK2 (green) and actin filaments (red) in epG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:Flow cytometric analysis of HepG2 cells with RSK2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human RSK2 recombinant protein, Hela, MCF-7, HepG2. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -8℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
