Application
Fig1: Western blot analysis of MCM2 on human MCM2 recombinant protein using anti-MCM2 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of MCM2 on different cell lysates using anti-MCM2 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Hela Lane 3: Jurkat Lane 4: K562 Lane 5: HEK293 Lane 6:HepG2Application
Fig3:ICC staining MCM2 (green) and actin filaments (red) in Hela cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using anti-MCM2 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer using anti-MCM2 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Jurkat cells with MCM2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | MCM2 [C5-E7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BM28 antibody CCNL 1 antibody CCNL1 antibody CDC like 1 antibody cdc19 antibody CDCL 1 antibody CDCL1 antibody Cell devision cycle like 1 antibody Cyclin like 1 antibody D3S3194 antibody DNA replication licensing factor MCM2 antibody KIAA0030 antibody MCM 2 antibody MCM2 antibody MCM2 minichromosome maintenance deficient 2 mitotin antibody MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody MCM2 minichromosome maintenance deficient 2, mitotin antibody MCM2_HUMAN antibody MGC10606 antibody Minichromosome maintenance complex component 2 antibody Minichromosome maintenance deficient 2 (mitotin) antibody Minichromosome maintenance deficient 2 mitotin antibody Minichromosome maintenance protein 2 antibody Minichromosome maintenance protein 2 homolog antibody Mitotin antibody Nuclear protein BM28 antibody OTTHUMP00000216047 antibody OTTHUMP00000216050 antibody |
| Product description | The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the post-replicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation during apoptosis of the MCM complex, which is composed of, at least, MCM2–6. A complex composed of MCM4, MCM6 and MCM7 has been shown to be involved in DNA helicase activity, and MCM5 is involved in IFN-γ-induced Stat1α transcription activation. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:100-1:500 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | BM28 antibody
CCNL 1 antibody
CCNL1 antibody
CDC like 1 antibody
cdc19 antibody
CDCL 1 antibody
CDCL1 antibody
Cell devision cycle like 1 antibody
Cyclin like 1 antibody
D3S3194 antibody
DNA replication licensing factor MCM2 antibody
KIAA0030 antibody
MCM 2 antibody
MCM2 antibody
MCM2 minichromosome maintenance deficient 2 mitotin antibody
MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody
MCM2 minichromosome maintenance deficient 2 mitotin antibody MCM2_HUMAN antibody MGC10606 antibody Minichromosome maintenance complex component 2 antibody Minichromosome maintenance deficient 2 (mitotin) antibody Minichromosome maintenance deficient 2 mitotin antibody Minichromosome maintenance protein 2 antibody Minichromosome maintenance protein 2 homolog antibody Mitotin antibody Nuclear protein BM28 antibody OTTHUMP00000216047 antibody OTTHUMP00000216050 antibody |
|---|---|
| Molecular Weight(MW) | 102 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P49736 |
|---|
Application
Fig1: Western blot analysis of MCM2 on human MCM2 recombinant protein using anti-MCM2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of MCM2 on different cell lysates using anti-MCM2 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Hela Lane 3: Jurkat Lane 4: K562 Lane 5: HEK293 Lane 6:HepG2
Application
Fig3:ICC staining MCM2 (green) and actin filaments (red) in Hela cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using anti-MCM2 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer using anti-MCM2 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Jurkat cells with MCM2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human MCM2 recombinant protein, MCF-7, Hela, Jurkat, K562, HEK293, HepG2, human ovarian cancer tissues, human colon cancer tissues. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:100-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
