Application
Fig1: Western blot analysis of PPP2R4 on human PPP2R4 recombinant protein using anti-PPP2R4 antibody at 1/1,000 dilution.Application
Fig2: ICC staining PPP2R4 (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded cervical cancer tissue using anti-PPP2R4 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded lung cancer tissue using anti-PPP2R4 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of MCF-7 cells with PPP2R4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | PPP2R4 [G4-D9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | subunit B antibody KIAA0044 antibody MGC2184 antibody Phosphotyrosyl phosphatase activator antibody PP2A antibody PP2A phosphatase activator antibody PP2A subunit B antibody PP2A subunit B isoform PR53 antibody PP2A subunit B PR53 isoform antibody PPP2R4 antibody PR53 antibody PR53 isoform antibody Protein phosphatase 2, regulatory subunit B-prime antibody Protein phosphatase 2A activator regulatory subunit 4 antibody Protein phosphatase 2A regulatory subunit B' (PR 53) antibody PTPA antibody PTPA_HUMAN antibody Serine/threonine protein phosphatase 2A regulatory subunit B antibody Serine/threonine-protein phosphatase 2A activator antibody Serine/threonine-protein phosphatase 2A regulatory subunit 4 antibody Serine/threonine-protein phosphatase 2A regulatory subunit B antibody |
| Product description | n general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. PPP2R4 (protein phosphatase 2A activator, regulatory subunit 4), also known as PR53 or PTPA, is a 358 amino acid protein that is widely expressed and associates with the PP holoenzyme. Specifically, PPP2R4 functions to stimulate the ATP- and magnesium-dependent phosphotyrosyl phosphatase activity of the dimeric form of PP2A, thereby affecting the control of cell growth and division. Four isoforms of PPP2R4, designated alpha, beta, delta and epsilon, are expressed due to alternative splicing events. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:100-1:500 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | subunit B antibody
KIAA0044 antibody
MGC2184 antibody
Phosphotyrosyl phosphatase activator antibody
PP2A antibody
PP2A phosphatase activator antibody
PP2A subunit B antibody
PP2A subunit B isoform PR53 antibody
PP2A subunit B PR53 isoform antibody
PPP2R4 antibody
PR53 antibody
PR53 isoform antibody
Protein phosphatase 2 regulatory subunit B-prime antibody Protein phosphatase 2A activator regulatory subunit 4 antibody Protein phosphatase 2A regulatory subunit B' (PR 53) antibody PTPA antibody PTPA_HUMAN antibody Serine/threonine protein phosphatase 2A regulatory subunit B antibody Serine/threonine-protein phosphatase 2A activator antibody Serine/threonine-protein phosphatase 2A regulatory subunit 4 antibody Serine/threonine-protein phosphatase 2A regulatory subunit B antibody |
|---|---|
| Molecular Weight(MW) | 41 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
| SwissProt ID | Q15257 |
|---|
Application
Fig1: Western blot analysis of PPP2R4 on human PPP2R4 recombinant protein using anti-PPP2R4 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining PPP2R4 (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded cervical cancer tissue using anti-PPP2R4 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded lung cancer tissue using anti-PPP2R4 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of MCF-7 cells with PPP2R4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human PPP2R4 recombinant protein, Hela, cervical cancer tissue, lung cancer tissue, MCF-7. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:100-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
