Application
Fig1: Western blot analysis of SERPINE1 on human SERPINE1 recombinant protein using anti-SERPINE1 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of SERPINE1 on HEK293 (1) and SERPINE1-hIgGFc transfected HEK293 (2) cell lysate using anti-SERPINE1 antibody at 1/1,000 dilution.Application
Fig3: Immunohistochemical analysis of paraffin-embedded lung cancer tissue using anti-SERPINE1 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded kidney cancer tissues using anti-SERPINE1 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of NIH/3T3 cells with SERPINE1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | SERPINE1 [H9-D5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Clade E antibody Endothelial plasminogen activator inhibitor antibody Nexin antibody PAI 1 antibody PAI antibody PAI-1 antibody PAI1_HUMAN antibody PLANH1 antibody Plasminogen activator inhibitor 1 antibody Plasminogen activator inhibitor type 1 antibody Serine (or cysteine) proteinase inhibitor antibody Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody Serpin E1 antibody Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody Serpin peptidase inhibitor clade E antibody Serpine 1 antibody SERPINE1 antibody |
| Product description | SERPINE1 is a protein of the serpin serine proteinase inhibitor family. It has shown to regulate uPA (urokinase-type plasminogen activator) and tPA (tissue plasminogen activator), resulting in the inhibition of proteolytic activity. Members of the serpin family generally complex with their target proteinases, then disassociate slowly into cleaved species that fold into stable inactive forms. uPA is a serine proteinase that is a member of the Trypsin family. It is responsible for the cleavage of plasminogen at the Arg-Val bond to produce plasmin. uPA consists of two chains designated A and B. The A chain can be cleaved, resulting in low and high molecular mass forms of uPA. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:1,000 IHC:1:100-1:500 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | Clade E antibody Endothelial plasminogen activator inhibitor antibody Nexin antibody PAI 1 antibody PAI antibody PAI-1 antibody PAI1_HUMAN antibody PLANH1 antibody Plasminogen activator inhibitor 1 antibody Plasminogen activator inhibitor type 1 antibody Serine (or cysteine) proteinase inhibitor antibody Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody Serpin E1 antibody Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody Serpin peptidase inhibitor clade E antibody Serpine 1 antibody SERPINE1 antibody |
|---|---|
| Molecular Weight(MW) | 45 kDa |
| Cellular Localization | Secreted. |
Application
Fig1: Western blot analysis of SERPINE1 on human SERPINE1 recombinant protein using anti-SERPINE1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of SERPINE1 on HEK293 (1) and SERPINE1-hIgGFc transfected HEK293 (2) cell lysate using anti-SERPINE1 antibody at 1/1,000 dilution.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded lung cancer tissue using anti-SERPINE1 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded kidney cancer tissues using anti-SERPINE1 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of NIH/3T3 cells with SERPINE1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human SERPINE1 recombinant protein, SERPINE1-hIgGFc transfected HEK293 cell lysate, lung cancer tissue, kidney cancer tissue, NIH/3T3. |
|---|---|
| Application Notes | WB:1:500-1:1,000 IHC:1:100-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
