Application
Fig1: Western blot analysis of CRTC1 on CRTC1-hIgGFc transfected HEK293 cell lysate using anti-CRTC1 antibody at 1/1,000 dilution.Application
Fig2: ICC staining CRTC1 (green) and actin filaments (red) in U251 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% TritonApplication
Fig3: ICC staining CRTC1 (green) and actin filaments (red) in NTERA2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4:Flow cytometric analysis of K562 cells with CRTC1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Product Name | CRTC1 [A2-B5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KIAA0616 antibody CREB regulated transcription coactivator 1 antibody CREB-regulated transcription coactivator 1 antibody CRTC1 antibody CRTC1_HUMAN antibody FLJ14027 antibody MECT 1 antibody Mucoepidermoid carcinoma translocated 1 antibody Mucoepidermoid carcinoma translocated protein 1 antibody TORC-1 antibody TORC1 antibody Transducer of CREB protein 1 antibody Transducer of regulated cAMP response element binding protein 1 antibody Transducer of regulated cAMP response element-binding protein (CREB) 1 antibody Transducer of regulated cAMP response element-binding protein 1 antibody WAMTP1 antibody |
| Product description | The TORC (transducer of regulated cAMP response element-binding) proteins, TORC1 and TORC2, are potent CREB coactivators that are exported from the nucleus in a CRM1-dependent manner. The translocation of TORC proteins is a conserved step in the activation of CRE-mediated gene expression induced by cAMP. TORC1 and TORC2 operate via phosphorylation-dependent interactions. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:1,000 ICC:1:100-1:500 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | KIAA0616 antibody CREB regulated transcription coactivator 1 antibody CREB-regulated transcription coactivator 1 antibody CRTC1 antibody CRTC1_HUMAN antibody FLJ14027 antibody MECT 1 antibody Mucoepidermoid carcinoma translocated 1 antibody Mucoepidermoid carcinoma translocated protein 1 antibody TORC-1 antibody TORC1 antibody Transducer of CREB protein 1 antibody Transducer of regulated cAMP response element binding protein 1 antibody Transducer of regulated cAMP response element-binding protein (CREB) 1 antibody Transducer of regulated cAMP response element-binding protein 1 antibody WAMTP1 antibody |
|---|---|
| Molecular Weight(MW) | 67 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
| SwissProt ID | Q6UUV9 |
|---|
Application
Fig1: Western blot analysis of CRTC1 on CRTC1-hIgGFc transfected HEK293 cell lysate using anti-CRTC1 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining CRTC1 (green) and actin filaments (red) in U251 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton
Application
Fig3: ICC staining CRTC1 (green) and actin filaments (red) in NTERA2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:Flow cytometric analysis of K562 cells with CRTC1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Positive Control | CRTC1-hIgGFc transfected HEK293 cell lysate, U251, NTERA2, K562. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:100-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
