Application
Fig1: Western blot analysis of ZBTB16 on Hela using anti-ZBTB16 antibody at 1/1,000 dilution.Application
Fig2: ICC staining ZBTB16 (green) and actin filaments (red) in Hela cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | ZBTB16 [E3-B3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AI467657 antibody lu antibody PLZF antibody Promyelocytic leukemia zinc finger protein antibody ZBT16_HUMAN antibody ZBTB16 antibody Zfp145 antibody Zinc finger and BTB domain containing 16 antibody zinc finger and BTB domain containing protein 16 antibody Zinc finger and BTB domain-containing protein 16 antibody Zinc finger protein 145 (Kruppel like expressed in promyelocytic leukemia) antibody Zinc finger protein 145 antibody Zinc finger protein PLZF antibody Znf145 antibody |
| Product description | Hypermethylated in cancer (HIC-1) was originally identified as a target of p53-induced gene expression. HIC-1 is deleted in the genetic disorder Miller-Dieker syndrome (MDS). The expression of HIC-1 is also frequently suppressed in leu-kemia and other various cancers due to the hypermethylation of specific DNA regions and the resulting transcriptional silencing. These and other studies indicate that HIC-1 acts as a putative tumor suppressor protein that mediates transcriptional repression. HIC-1 is ubiquitously expressed in adult tissues and its structure is defined by five zinc fingers and an N-terminal broad-complex POZ (or BTB) domain. The BTB/POZ domain mediates homomeric and heteromeric POZ-POZ interactions and is common to transcriptional regulators involved in chromatin modeling. In several BTB/POZ containing proteins, including BCL-6 and the promyelocytic leukemia zinc finger (PLZF) oncoprotein, this domain interacts with the SMRT/N-CoR-mSin3A HDAC complex and is directly involved in repressing and silencing gene transcription. When this domain is deleted, as with the oncogenic PLZF-RAR chimera of promyelocytic leukemias, this transcriptional repression is attenuated. Conversely, HIC-1 does not interact with components of the HDAC complex, suggesting that HIC-1-induced transcriptional repression is unassociated with the POZ/BTB domain. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICC |
| WB:1:500-1:2,000 ICC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | AI467657 antibody lu antibody PLZF antibody Promyelocytic leukemia zinc finger protein antibody ZBT16_HUMAN antibody ZBTB16 antibody Zfp145 antibody Zinc finger and BTB domain containing 16 antibody zinc finger and BTB domain containing protein 16 antibody Zinc finger and BTB domain-containing protein 16 antibody Zinc finger protein 145 (Kruppel like expressed in promyelocytic leukemia) antibody Zinc finger protein 145 antibody Zinc finger protein PLZF antibody Znf145 antibody |
|---|---|
| Molecular Weight(MW) | 74 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q05516 |
|---|
Application
Fig1: Western blot analysis of ZBTB16 on Hela using anti-ZBTB16 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining ZBTB16 (green) and actin filaments (red) in Hela cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
