Application
Fig1: Western blot analysis of Rab10 on Hela (1) and NIH/3T3 (2) cell lysate using anti-Rab10 antibody at 1/1,000 dilution.Application
Fig2: ICC staining Rab10 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | Rab10 [F8-E2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GTP binding protein RAB10 antibody Rab10 antibody RAB10 member RAS oncogene family antibody RAB10_HUMAN antibody Ras related GTP binding protein antibody Ras related GTP binding protein RAB10 antibody Ras-related protein Rab-10 antibody |
| Product description | The Ras-related superfamily of guanine nucleotide binding proteins, which includes the Ral / Rec, Rap, R-Ras, and Rho / Rab subfamilies, exhibit 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. Several members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICC |
| WB:1:500-1:1,000 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | GTP binding protein RAB10 antibody Rab10 antibody RAB10 member RAS oncogene family antibody RAB10_HUMAN antibody Ras related GTP binding protein antibody Ras related GTP binding protein RAB10 antibody Ras-related protein Rab-10 antibody |
|---|---|
| Molecular Weight(MW) | 23 kDa |
| Cellular Localization | Cytoplasmic vesicle membrane. Cytosol. |
Application
Fig1: Western blot analysis of Rab10 on Hela (1) and NIH/3T3 (2) cell lysate using anti-Rab10 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Rab10 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | Hela, NIH-3T3, LOVO.Hela, NIH-3T3, LOVO. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
