Application
Fig1: Western blot analysis of Thrombopoietin on human Thrombopoietin recombinant protein using anti- Thrombopoietin antibody at 1/1,000 dilution.Application
Fig2: Flow cytometric analysis of MCF-7 cells with Thrombopoietin antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | THPO [C10-B11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C mpl ligand antibody C-mpl ligand antibody Megakaryocyte colony stimulating factor antibody Megakaryocyte colony-stimulating factor antibody Megakaryocyte growth and development factor antibody Megakaryocyte stimulating factor antibody MGC163194 antibody MGDF antibody MKCSF antibody ML antibody MPL ligand antibody MPLLG antibody Myeloproliferative leukemia virus oncogene ligand antibody Prepro thrombopoietin antibody THCYT1 antibody THPO antibody Thrombopoietin antibody Thrombopoietin nirs variant 1 antibody TPO antibody TPO_HUMAN antibody |
| Product description | Thrombopoietin (TPO or THPO), also known as c-Mpl ligand (c-Mpl L), is a cytokine that plays a central role in megakaryopoiesis by influencing the development and maturation of megakaryocytes and platelet production. TPO is expressed by both mature and progenitor megakaryocytes, as well as by human platelets. Human TPO cDNA encodes a 332 amino acid precursor with a 21 amino acid signal peptide which is cleaved to generate the mature protein. TPO isolated from serum ranges in molecular weight, which suggests that the protein is highly glycosylated. TPO exerts its biological effects through the TPO receptor, c-Mpl. Stimulation of c-Mpl with TPO results in the activation of the Janus tyrosine kinase family members, The gene encoding TPO maps to human chromosome 3q26.3-q27. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:1,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | C mpl ligand antibody C-mpl ligand antibody Megakaryocyte colony stimulating factor antibody Megakaryocyte colony-stimulating factor antibody Megakaryocyte growth and development factor antibody Megakaryocyte stimulating factor antibody MGC163194 antibody MGDF antibody MKCSF antibody ML antibody MPL ligand antibody MPLLG antibody Myeloproliferative leukemia virus oncogene ligand antibody Prepro thrombopoietin antibody THCYT1 antibody THPO antibody Thrombopoietin antibody Thrombopoietin nirs variant 1 antibody TPO antibody TPO_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 38 kDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P40225 |
|---|
Application
Fig1: Western blot analysis of Thrombopoietin on human Thrombopoietin recombinant protein using anti- Thrombopoietin antibody at 1/1,000 dilution.
Application
Fig2: Flow cytometric analysis of MCF-7 cells with Thrombopoietin antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human THPO recombinant protein, MCF-7. |
|---|---|
| Application Notes | WB:1:500-1:1,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
