Application
Fig1: Western blot analysis of WIF1 on different cell lysates using anti-WIF1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: NTERA-2Application
Fig2: ICC staining WIF1 (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human ovary tumour tissue using anti-WIF1 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-WIF1 antibody. Counter stained with hematoxylin.| Product Name | WIF1 [E7-G5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WIF-1 antibody wif1 antibody WIF1_HUMAN antibody Wnt inhibitory factor 1 antibody |
| Product description | The Wnt genes are a group of conserved, Cysteine-rich, secreted glycoproteins that are required for numerous developmental processes including embryogenesis, asymmetric cell division and central nervous system (CNS) patterning. Wnt association with the transmembrane spanning receptor frizzled activates dishevelled, which downregulates glycogen synthase kinase (GSK) through serine phosphorylation. Reduced levels of active GSK causes accumulation of b-catenin and subsequent regulation of developmentally significant Wnt target genes. Wnt antagonists such as dickkopf (Dkk), frizzled-related protein (sFRP) and Wnt inhibitory factor-1 (WIF-1), are necessary to ensure normal spatial and temporal patterns of Wnt activity during developmental processes. WIF-1 is a 379-amino acid secreted protein that contains an N-terminal signal sequence, a 150-amino acid WIF domain, five epidermal growth factor-like repeats and a 45-amino acid C-terminal hydrophilic domain. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHC |
| WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | WIF-1 antibody wif1 antibody WIF1_HUMAN antibody Wnt inhibitory factor 1 antibody |
|---|---|
| Molecular Weight(MW) | 42 kDa |
| Cellular Localization | Secreted. |
Application
Fig1: Western blot analysis of WIF1 on different cell lysates using anti-WIF1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: NTERA-2
Application
Fig2: ICC staining WIF1 (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human ovary tumour tissue using anti-WIF1 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-WIF1 antibody. Counter stained with hematoxylin.| Positive Control | Hela, NIH/3T3, NTERA-2, human ovary tumour tissue, human lung cancer tissue. |
|---|---|
| Application Notes | WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
