Application
Fig1: Western blot analysis of RICTOR on different cell lysates using anti-RICTOR antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PANC-1 Lane 3: MOLT4 Lane 4: HepG2 Lane 5: HEK293Application
Fig2: ICC staining RICTOR (green) and actin filaments (red) in NIH/3T3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-RICTOR antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-RICTOR antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of Hela cells with RICTOR antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Product Name | RICTOR [E9-B3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AVO3 antibody AVO3 homolog antibody DKFZp686B11164 antibody hAVO3 antibody KIAA1999 antibody Likely ortholog of mouse TORC2 specific protein AVO3 (S. cerevisiae) antibody mAVO3 antibody MGC39830 antibody PIA antibody Pianissimo antibody Rapamycin insensitive companion of mTOR antibody Rapamycin-insensitive companion of mTOR antibody Rictor antibody RICTR antibody RICTR_HUMAN antibody RPTOR independent companion of MTOR complex 2 antibody TORC2 specific protein AVO3 antibody |
| Product description | Rapamycin-insensitive companion of FRAP, also designated Rictor, shares homology with pianissimo from D. discoidieum, STE20p from S . pombe, and AVO3p from S. cerevisiae. Rictor forms a complex with FRAP, which is important in cell growth regulation as it integrates growth factor and nutrient derived signals. The Rictor-FRAP complex plays a role in PKC alpha phosphorylation, directly phosphorylates Akt / PKB on Ser473 in vitro and facilitates Thr308 phosphorylation by PDK1. It also may function as a drug target in tumors that have lost expression of PTEN, a tumor suppressor that opposes activation of Akt / PKB. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouseMonkey |
| Concentration | 2mg/ml |
| Alternative Names | AVO3 antibody AVO3 homolog antibody DKFZp686B11164 antibody hAVO3 antibody KIAA1999 antibody Likely ortholog of mouse TORC2 specific protein AVO3 (S. cerevisiae) antibody mAVO3 antibody MGC39830 antibody PIA antibody Pianissimo antibody Rapamycin insensitive companion of mTOR antibody Rapamycin-insensitive companion of mTOR antibody Rictor antibody RICTR antibody RICTR_HUMAN antibody RPTOR independent companion of MTOR complex 2 antibody TORC2 specific protein AVO3 antibody |
|---|---|
| Molecular Weight(MW) | 192 kDa |
| Cellular Localization | Cytosol. |
Application
Fig1: Western blot analysis of RICTOR on different cell lysates using anti-RICTOR antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PANC-1 Lane 3: MOLT4 Lane 4: HepG2 Lane 5: HEK293
Application
Fig2: ICC staining RICTOR (green) and actin filaments (red) in NIH/3T3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-RICTOR antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-RICTOR antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of Hela cells with RICTOR antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Positive Control | Hela, PANC-1, MOLT4, HepG2, HEK293, NIH-3T3, human thyroid gland tissue, human breast carcinoma tissue. |
|---|---|
| Application Notes | WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
