Application
Fig1: Western blot analysis of RPA70 on human RPA70 recombinant protein using anti-RPA70 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of RPA70 on different cell lysates using anti-RPA70 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: K562 Lane 4: A431 Lane 5: COS-7Application
Fig3: ICC staining RPA70 (green) and actin filaments (red) in Hela cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded colon cancer tissue using anti-RPA70 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded prostate tissue using anti-RPA70 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Jurkat cells with RPA70 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | RPA1 [D9-C4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Dmrpa1 antibody Drosophila Replication Protein A antibody DRPA antibody HSSB antibody Human single stranded DNA binding protein antibody MST075 antibody MSTP075 antibody p70 antibody REPA1 antibody Replication factor A antibody Replication factor A protein 1 antibody Replication protein A 70 kDa DNA-binding subunit antibody Replication protein A 70kDa DNA binding subunit antibody Replication protein A1 70kDa antibody Replication protein A1 antibody RF A antibody RF-A protein 1 antibody RFA antibody RFA1_HUMAN antibody RP A antibody RP-A p70 antibody RPA 70 antibody RPA antibody rpa1 antibody Single stranded binding protein 70 antibody Single-stranded DNA-binding protein antibody |
| Product description | RPA plays an important role in DNA replication, recombination and repair. The binding of human RPA (hRPA) to DNA involves molecular polarity in which initial hRPA binding occurs on the 5' side of an ssDNA substrate and then extends in the 3' direction to create a stably bound hRPA. RPA is a major damage-recognition protein involved in the early stages of nucleotide excision repair. It can also play a role in telomere maintenance. The RPA 70 kDa subunit binds to ssDNA and mediates interactions with many cellular and viral proteins. The DNA binding domain lies in the middle of RPA 70 kDa subunit and comprises two structurally homologous subdomains oriented in tandem. RPA contains a conserved four cysteine-type zinc-finger motif, which mediates the transition of RPA-ssDNA interaction to a stable RPA-ssDNA complex in a redox-dependent manner. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:100-1:500 IHC:1:100-1:500 FC:1:100-1:200 |
|
| Species Reactivity | HumanMonkey |
| Concentration | 2mg/ml |
| Alternative Names | Dmrpa1 antibody Drosophila Replication Protein A antibody DRPA antibody HSSB antibody Human single stranded DNA binding protein antibody MST075 antibody MSTP075 antibody p70 antibody REPA1 antibody Replication factor A antibody Replication factor A protein 1 antibody Replication protein A 70 kDa DNA-binding subunit antibody Replication protein A 70kDa DNA binding subunit antibody Replication protein A1 70kDa antibody Replication protein A1 antibody RF A antibody RF-A protein 1 antibody RFA antibody RFA1_HUMAN antibody RP A antibody RP-A p70 antibody RPA 70 antibody RPA antibody rpa1 antibody Single stranded binding protein 70 antibody Single-stranded DNA-binding protein antibody |
|---|---|
| Molecular Weight(MW) | 70 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P27694 |
|---|
Application
Fig1: Western blot analysis of RPA70 on human RPA70 recombinant protein using anti-RPA70 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of RPA70 on different cell lysates using anti-RPA70 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: K562 Lane 4: A431 Lane 5: COS-7
Application
Fig3: ICC staining RPA70 (green) and actin filaments (red) in Hela cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded colon cancer tissue using anti-RPA70 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded prostate tissue using anti-RPA70 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Jurkat cells with RPA70 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human RPA1 recombinant protein, Hela, MCF-7, K562, A431, COS-7, colon cancer tissue, prostate tissue, Jurkat. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:100-1:500 IHC:1:100-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
