Application
Fig1: Western blot analysis of PSMB8 on human PSMB8 recombinant protein using anti-PSMB8 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of PSMB8 on different cell lysates using anti-PSMB8 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: A431 Lane 4: RAJI Lane 5: MOTL4 Lane 6: PC-12Application
Fig3: ICC staining PSMB8 (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded intima cancer tissue using anti-PSMB8 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-PSMB8 antibody. Counter stained with hematoxylin.| Product Name | PSMB8 [H2-A5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ALDD antibody D6S216 antibody D6S216E antibodyLarge multifunctional peptidase 7 antibody Large multifunctional protease 7 antibody LMP 7 antibody LMP7 antibody Low molecular mass protein 7 antibody Low molecular weight protein 7 antibody Macropain subunit C13 antibody MGC1491 antibody Multicatalytic endopeptidase complex subunit C13 antibody NKJO antibody OTTHUMP00000062981 antibody Protease component C13 antibody Proteasome (prosome macropain) subunit beta type 8 antibody Proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7) antibody Proteasome beta 8 subunit antibody Proteasome catalytic subunit 3i antibody Proteasome component C13 antibody Proteasome related gene 7 antibody Proteasome subunit beta 5i antibody Proteasome subunit beta 8 antibody Proteasome subunit beta type 8 antibody Proteasome subunit beta type antibody Proteasome subunit beta type-8 antibody Proteasome subunit beta-5i antibody Proteasome subunit Y2 antibody PSB8_HUMAN antibody PSMB 8 antibody PSMB5i antibody PSMB8 antibody Really interesting new gene 10 protein antibody RING 10 antibody RING10 antibody Y2 antibody |
| Product description | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHC |
| WB:1:500-1:2,000 ICC:1:100-1:500 IHC:1:100-1:500 |
|
| Species Reactivity | HumanRat |
| Concentration | 2mg/ml |
| Alternative Names | ALDD antibody
D6S216 antibody
D6S216E antibodyLarge multifunctional peptidase 7 antibody
Large multifunctional protease 7 antibody
LMP 7 antibody
LMP7 antibody
Low molecular mass protein 7 antibody
Low molecular weight protein 7 antibody
Macropain subunit C13 antibody
MGC1491 antibody
Multicatalytic endopeptidase complex subunit C13 antibody
NKJO antibody
OTTHUMP00000062981 antibody
Protease component C13 antibody
Proteasome (prosome macropain) subunit beta type 8 antibody
Proteasome (prosome macropain) subunit beta type 8 (large multifunctional peptidase 7) antibody Proteasome beta 8 subunit antibody Proteasome catalytic subunit 3i antibody Proteasome component C13 antibody Proteasome related gene 7 antibody Proteasome subunit beta 5i antibody Proteasome subunit beta 8 antibody Proteasome subunit beta type 8 antibody Proteasome subunit beta type antibody Proteasome subunit beta type-8 antibody Proteasome subunit beta-5i antibody Proteasome subunit Y2 antibody PSB8_HUMAN antibody PSMB 8 antibody PSMB5i antibody PSMB8 antibody Really interesting new gene 10 protein antibody RING 10 antibody RING10 antibody Y2 antibody |
|---|---|
| Molecular Weight(MW) | 22 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
Application
Fig1: Western blot analysis of PSMB8 on human PSMB8 recombinant protein using anti-PSMB8 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PSMB8 on different cell lysates using anti-PSMB8 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: A431 Lane 4: RAJI Lane 5: MOTL4 Lane 6: PC-12
Application
Fig3: ICC staining PSMB8 (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded intima cancer tissue using anti-PSMB8 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-PSMB8 antibody. Counter stained with hematoxylin.| Application Notes | WB:1:500-1:2,000 ICC:1:100-1:500 IHC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
