Application
Fig1: Western blot analysis of SMAD6 on different cell lysates using anti-SMAD6 antibody at 1/1,000 dilution. Positive control： Lane1: A431 Lane2: A431 Lane3: Hela Lane4: Jurkat| Product Name | SMAD6 [H6-H3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | hSMAD6 antibody MAD homolog 6 antibody MADH6 antibody MADH7 antibody Mothers against decapentaplegic homolog 6 antibody Mothers against DPP homolog 6 antibody SMAD 6 antibody SMAD family member 6 antibody SMAD mothers against DPP homolog 6 antibody Smad6 antibody SMAD6_HUMAN antibody |
| Product description | Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation (2-5,8). Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500-1:1,000 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | hSMAD6 antibody MAD homolog 6 antibody MADH6 antibody MADH7 antibody Mothers against decapentaplegic homolog 6 antibody Mothers against DPP homolog 6 antibody SMAD 6 antibody SMAD family member 6 antibody SMAD mothers against DPP homolog 6 antibody Smad6 antibody SMAD6_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 53 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | O43541 |
|---|
Application
Fig1: Western blot analysis of SMAD6 on different cell lysates using anti-SMAD6 antibody at 1/1,000 dilution. Positive control： Lane1: A431 Lane2: A431 Lane3: Hela Lane4: Jurkat| Positive Control | A431, Hela, Jurkat. |
|---|---|
| Application Notes | WB:1:500-1:1,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
