Application
Fig1: Western blot analysis of Glypican3 on human Glypican3 recombinant protein using anti- Glypican3 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of Glypican3 on different lysates using anti- Glypican3 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: HEK293 Lane 3:Jurkat Lane 4: SK-N-SH Lane 5: PC-12 Lane 6: F9 Lane 7: Mouse liver tissueApplication
Fig3: ICC staining Glypican3 (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded liver cancer tissue using anti- Glypican3 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded breast cancer tissue using anti- Glypican3 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Jurkat cells with Glypican3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | GPC3 [G3-C2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DGSX antibody Glypican proteoglycan 3 antibody Glypican-3 [Precursor] antibody Gpc3 antibody GPC3_HUMAN antibody GTR2 2 antibody GTR2-2 antibody Heparan sulphate proteoglycan antibody Intestinal protein OCI 5 antibody Intestinal protein OCI-5 antibody MXR7 antibody OCI 5 antibody OCI-5 antibody OCI5 antibody SDYS antibody Secreted glypican-3 antibody SGB antibody SGBS antibody SGBS1 antibody |
| Product description | Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:100-1:500 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | DGSX antibody Glypican proteoglycan 3 antibody Glypican-3 [Precursor] antibody Gpc3 antibody GPC3_HUMAN antibody GTR2 2 antibody GTR2-2 antibody Heparan sulphate proteoglycan antibody Intestinal protein OCI 5 antibody Intestinal protein OCI-5 antibody MXR7 antibody OCI 5 antibody OCI-5 antibody OCI5 antibody SDYS antibody Secreted glypican-3 antibody SGB antibody SGBS antibody SGBS1 antibody |
|---|---|
| Molecular Weight(MW) | 65 kDa |
| Cellular Localization | Plasma membrane. Secreted. |
Application
Fig1: Western blot analysis of Glypican3 on human Glypican3 recombinant protein using anti- Glypican3 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of Glypican3 on different lysates using anti- Glypican3 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: HEK293 Lane 3:Jurkat Lane 4: SK-N-SH Lane 5: PC-12 Lane 6: F9 Lane 7: Mouse liver tissue
Application
Fig3: ICC staining Glypican3 (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded liver cancer tissue using anti- Glypican3 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded breast cancer tissue using anti- Glypican3 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Jurkat cells with Glypican3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human GPC3 recombinant protein, HepG2, HEK293, SK-N-SH, PC-12, F9, HeLa, liver cancer tissue, breast cancer tissue, Jurkat. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:100-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
