Application
Fig1: Western blot analysis of ITK on human ITK recombinant protein using anti-ITK antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of ITK on Jurkat cell lysate using anti-ITK antibody at 1/1,000 dilution.Application
Fig3: ICC staining ITK (green) in Jurkat cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Flow cytometric analysis of Jurkat cells with ITK antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | ITK [A6-G12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EMT antibody Homolog of mouse T cell itk/tsk antibody IL 2 inducible T cell kinase antibody IL2 inducible T cell kinase antibody Interleukin 2 inducible T cell kinase antibody Itk antibody ITK_HUMAN antibody Kinase EMT antibody LPFS1 antibody LYK antibody MGC126257 antibody MGC126258 antibody PSCTK 2 antibody PSCTK2 antibody T cell specific kinase antibody T-cell-specific kinase antibody TSK antibody Tyrosine protein kinase antibody Tyrosine protein kinase ITK/TSK antibody Tyrosine protein kinase Lyk antibody Tyrosine-protein kinase ITK/TSK antibody Tyrosine-protein kinase Lyk antibody |
| Product description | The Tec family of non-receptor tyrosine kinases is composed of six proteins designated Tec, Emt (also known as Itk or Tsk), Btk (previously known as Atk, BPK or Emb), Bmx, Txk (also known as Rlk) and Dsrc28C. Four alternatively spliced forms of Tec are found to be expressed broadly in cells Emt gene product associates with CD28 and becomes activated subsequent to CD28 ligation. Btk is necessary for proper B cell development, and mutations in the gene encoding Btk have been associated with families suffering from X-linked agammaglobulinemia, also referred to as Bruton's disease. The Bmx protein shares a high degree of homology with Btk and seems to be expressed at highest levels in the heart. Txk expression is T cell-specific, while expression of the Drosophila Tec homolog, Dsrc28C, is developmentally regulated. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | EMT antibody Homolog of mouse T cell itk/tsk antibody IL 2 inducible T cell kinase antibody IL2 inducible T cell kinase antibody Interleukin 2 inducible T cell kinase antibody Itk antibody ITK_HUMAN antibody Kinase EMT antibody LPFS1 antibody LYK antibody MGC126257 antibody MGC126258 antibody PSCTK 2 antibody PSCTK2 antibody T cell specific kinase antibody T-cell-specific kinase antibody TSK antibody Tyrosine protein kinase antibody Tyrosine protein kinase ITK/TSK antibody Tyrosine protein kinase Lyk antibody Tyrosine-protein kinase ITK/TSK antibody Tyrosine-protein kinase Lyk antibody |
|---|---|
| Molecular Weight(MW) | 72 kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | Q08881 |
|---|
Application
Fig1: Western blot analysis of ITK on human ITK recombinant protein using anti-ITK antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of ITK on Jurkat cell lysate using anti-ITK antibody at 1/1,000 dilution.
Application
Fig3: ICC staining ITK (green) in Jurkat cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Flow cytometric analysis of Jurkat cells with ITK antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human ITK recombinant protein, Jurkat. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
