Application
Fig1: Western blot analysis of UBE1L on Raji (1) and THP-1 (2) cell lysate using anti-UBE1L antibody at 1/1,000 dilution.| Product Name | UBE1L [A1-B10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | D8 antibody MGC12713 antibody UBA1, ubiquitin activating enzyme E1 homolog B antibody UBA1B antibody UBA7 antibody UBA7, ubiquitin activating enzyme E1 antibody UBA7_HUMAN antibody Ube 1L antibody UBE 2 antibody UBE1L antibody UBE2 antibody Ubiquitin activating enzyme 2 antibody Ubiquitin activating enzyme E1 homolog antibody Ubiquitin Activating Enzyme E1 Like antibody Ubiquitin activating enzyme E1 related protein antibody Ubiquitin-activating enzyme 7 antibody Ubiquitin-activating enzyme E1 homolog antibody ubiquitin-activating enzyme-2 antibody ubiquitin-like modifier activating enzyme 7 antibody Ubiquitin-like modifier-activating enzyme 7 antibody |
| Product description | The ubiquitin activating enzyme E1 (UBE1) catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. UBE1 activates ubiquitin by first adenylating (with ATP) its carboxy-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and a free AMP. UBE1 is an example of an X-Y homologous gene, which is X-linked with a distinct Y-linked gene in many mammals. UBE1L (Ubiquitin-activating enzyme E1 homolog), also known as UBA7 (Ubiquitin-like modifier-activating enzyme 7) or UBE2, is a 1011 amino acid homolog of UBE1. Like UBE1, UBE1L functions in the activation of ubiquitin through ATP-dependent adenylation. UBE1L is expressed in tumor cells and is a retinoid target that, through conjugation with ISG15 (Interferon-induced 15 kDa protein), triggers degradation and apoptosis in acute promyelocytic leukemia. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500-1:1,000 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | D8 antibody
MGC12713 antibody
UBA1 ubiquitin activating enzyme E1 homolog B antibody UBA1B antibody UBA7 antibody UBA7 ubiquitin activating enzyme E1 antibody UBA7_HUMAN antibody Ube 1L antibody UBE 2 antibody UBE1L antibody UBE2 antibody Ubiquitin activating enzyme 2 antibody Ubiquitin activating enzyme E1 homolog antibody Ubiquitin Activating Enzyme E1 Like antibody Ubiquitin activating enzyme E1 related protein antibody Ubiquitin-activating enzyme 7 antibody Ubiquitin-activating enzyme E1 homolog antibody ubiquitin-activating enzyme-2 antibody ubiquitin-like modifier activating enzyme 7 antibody Ubiquitin-like modifier-activating enzyme 7 antibody |
|---|---|
| Molecular Weight(MW) | 112 kDa |
| Cellular Localization | Nucleus. Cytosol. |
| SwissProt ID | P41226 |
|---|
Application
Fig1: Western blot analysis of UBE1L on Raji (1) and THP-1 (2) cell lysate using anti-UBE1L antibody at 1/1,000 dilution.| Positive Control | Raji, THP-1. |
|---|---|
| Application Notes | WB:1:500-1:1,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
