Application
Fig1: Western blot analysis of MAP2K4 on different cell lysates using anti-MAP2K4 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: K562 Lane 3: HEK293Application
Fig2: Immunohistochemical analysis of paraffin-embedded human muscle tissue using anti-MAP2K4 antibody. Counter stained with hematoxylin.Application
Fig3: Flow cytometric analysis of K562 cells with MAP2K4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Product Name | MAP2K4 [B4-H6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | c Jun N terminal kinase kinase 1 antibody C-JUN N-terminal kinase kinase 1 antibody Dual specificity mitogen activated protein kinase kinase 4 antibody Dual specificity mitogen-activated protein kinase kinase 4 antibody JNK Activated Kinase 1 antibody JNK activating kinase 1 antibody JNK-activating kinase 1 antibody JNKK antibody JNKK1 antibody MAP kinase kinase 4 antibody Map2k4 antibody MAPK ERK kinase 4 antibody MAPK/ERK kinase 4 antibody MAPKK 4 antibody MAPKK4 antibody MEK 4 antibody MEK4 antibody Mitogen activated protein kinase kinase 4 antibody MKK 4 antibody MKK4 antibody MP2K4_HUMAN antibody PRKMK4 antibody SAPK ERK kinase 1 antibody SAPK/ERK kinase 1 antibody SAPKK 1 antibody SAPKK1 antibody SEK1 antibody SERK1 antibody SKK1 antibody Stress activated protein kinase kinase 1 antibody |
| Product description | A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:1,000 IHC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | c Jun N terminal kinase kinase 1 antibody C-JUN N-terminal kinase kinase 1 antibody Dual specificity mitogen activated protein kinase kinase 4 antibody Dual specificity mitogen-activated protein kinase kinase 4 antibody JNK Activated Kinase 1 antibody JNK activating kinase 1 antibody JNK-activating kinase 1 antibody JNKK antibody JNKK1 antibody MAP kinase kinase 4 antibody Map2k4 antibody MAPK ERK kinase 4 antibody MAPK/ERK kinase 4 antibody MAPKK 4 antibody MAPKK4 antibody MEK 4 antibody MEK4 antibody Mitogen activated protein kinase kinase 4 antibody MKK 4 antibody MKK4 antibody MP2K4_HUMAN antibody PRKMK4 antibody SAPK ERK kinase 1 antibody SAPK/ERK kinase 1 antibody SAPKK 1 antibody SAPKK1 antibody SEK1 antibody SERK1 antibody SKK1 antibody Stress activated protein kinase kinase 1 antibody |
|---|---|
| Molecular Weight(MW) | 44 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
| SwissProt ID | P45985 |
|---|
Application
Fig1: Western blot analysis of MAP2K4 on different cell lysates using anti-MAP2K4 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: K562 Lane 3: HEK293
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human muscle tissue using anti-MAP2K4 antibody. Counter stained with hematoxylin.
Application
Fig3: Flow cytometric analysis of K562 cells with MAP2K4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Positive Control | HepG2, K562, HEK293, human muscle tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
