Application
Fig1: Western blot analysis of HFE on human HFE recombinant protein using anti-HFE antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of HFE on HEK293 (1) and HFE-hIgGFc transfected HEK293 (2) cell lysate using anti-HFE antibody at 1/1,000 dilution.Application
Fig3: ICC staining HFE (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | HFE [D10-F1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | dJ221C16.10.1 antibody Hemochromatosis antibody Hemochromatosis protein antibody Hereditary hemochromatosis protein antibody Hereditary hemochromatosis protein HLA H antibody HFE 1 antibody HFE antibody HFE_HUMAN antibody HFE1 antibody HH antibody High Fe antibody HLA H antibody HLA-H antibody HLAH antibody MGC:150812 antibody MGC10379 antibody MGC103790 antibody MHC class I like protein HFE antibody MVCD7 antibody TFQTL2 antibody |
| Product description | The HFE protein, which is defective in hereditary hemo-chromatosis, normally is expressed in crypt enterocytes of the duodenum where it has a unique, predominantly intracellular localization. In placenta, the HFE protein co-localizes with and forms a stable association with the transferrin receptor (TfR), providing a link between the HFE protein and iron transport. Immunocytochemistry shows that the HFE protein and TfR both are expressed in the crypt enterocytes. It is proposed that HFE has two mutually exclusive activities in cells: inhibition of uptake or inhibition of release of iron and that the balance between serum transferrin saturation and serum transferrin-receptor concentrations determines which of these functions predominates. The gene which encodes HFE maps to human chromosome 6p21.3. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICC |
| WB:1:500-1:1,000 ICC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | dJ221C16.10.1 antibody Hemochromatosis antibody Hemochromatosis protein antibody Hereditary hemochromatosis protein antibody Hereditary hemochromatosis protein HLA H antibody HFE 1 antibody HFE antibody HFE_HUMAN antibody HFE1 antibody HH antibody High Fe antibody HLA H antibody HLA-H antibody HLAH antibody MGC:150812 antibody MGC10379 antibody MGC103790 antibody MHC class I like protein HFE antibody MVCD7 antibody TFQTL2 antibody |
|---|---|
| Molecular Weight(MW) | 40 kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | Q30201 |
|---|
Application
Fig1: Western blot analysis of HFE on human HFE recombinant protein using anti-HFE antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of HFE on HEK293 (1) and HFE-hIgGFc transfected HEK293 (2) cell lysate using anti-HFE antibody at 1/1,000 dilution.
Application
Fig3: ICC staining HFE (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | Human HFE recombinant protein, HFE-hIgGFc transfected HEK293 cell lysate, HepG2. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
