Application
Fig1: Western blot analysis of TCP1 beta on human TCP1 beta recombinant protein using anti- TCP1 beta antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of TCP1 beta on different cell lysates using anti- TCP1 beta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: Jurkat Lane 4: T47D Lane 5: K562 Lane 6: A431 Lane 7: NIH/3T3 Lane 8: PC-12 Lane 9: Cos7Application
Fig3: Flow cytometric analysis of NIH/3T3 cells with TCP1 beta antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | CCT2 [C4-B5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 99D8.1 antibody CCT 2 antibody CCT beta antibody CCT-beta antibody CCT2 antibody CCTB antibody Chaperonin containing t complex polypeptide 1 beta subunit antibody Chaperonin containing t complex polypeptide 1 subunit 2 antibody Chaperonin containing TCP1 subunit 2 antibody Chaperonin containing TCP1 subunit 2 (beta) antibody CTP:phosphocholine cytidylyltransferase 2 antibody Epididymis secretory sperm binding protein Li 100n antibody HEL S 100n antibody MGC142074 antibody MGC142076 antibody MGC94480 antibody PRO1633 antibody T complex protein 1 beta subunit antibody T complex protein 1 subunit beta antibody T-complex protein 1 subunit beta antibody TCP 1 beta antibody TCP-1-beta antibody TCPB_HUMAN antibody |
| Product description | The protein TCP-1 (t complex polypeptide 1) is a subunit of the hetero-oligomeric complex CCT (chaperonin containing TCP-1) present in the eukaryotic cytosol. The CCT of eukaryotic cytosol is composed of eight different subunit species, TCP-1 α, β, γ, δ, ε, ζ, η and θ, each encoded by a different gene. TCP-1 subunits are proposed to have independent functions in folding its in vivo substrates, the actins and tubulins. TCP-1 was first identified in the mouse as relevant for tail-less and embryonic lethal phenotypes. Sequences homologous to TCP-1 have been isolated in several other species, and the yeast TCP-1 has been shown to encode a molecular chaperone for Actin and Tubulin. TCP-1 found in mammalian cells and yeast plays an important role in the folding of cytosolic proteins. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:1,000 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouseRatMonkey |
| Concentration | 2mg/ml |
| Alternative Names | 99D8.1 antibody CCT 2 antibody CCT beta antibody CCT-beta antibody CCT2 antibody CCTB antibody Chaperonin containing t complex polypeptide 1 beta subunit antibody Chaperonin containing t complex polypeptide 1 subunit 2 antibody Chaperonin containing TCP1 subunit 2 antibody Chaperonin containing TCP1 subunit 2 (beta) antibody CTP:phosphocholine cytidylyltransferase 2 antibody Epididymis secretory sperm binding protein Li 100n antibody HEL S 100n antibody MGC142074 antibody MGC142076 antibody MGC94480 antibody PRO1633 antibody T complex protein 1 beta subunit antibody T complex protein 1 subunit beta antibody T-complex protein 1 subunit beta antibody TCP 1 beta antibody TCP-1-beta antibody TCPB_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 54 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of TCP1 beta on human TCP1 beta recombinant protein using anti- TCP1 beta antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of TCP1 beta on different cell lysates using anti- TCP1 beta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: Jurkat Lane 4: T47D Lane 5: K562 Lane 6: A431 Lane 7: NIH/3T3 Lane 8: PC-12 Lane 9: Cos7
Application
Fig3: Flow cytometric analysis of NIH/3T3 cells with TCP1 beta antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human TCP1 beta recombinant protein, Hela, MCF-7, Jurkat, T47D, K562, A431, NIH/3T3, PC-12, Cos7. |
|---|---|
| Application Notes | WB:1:500-1:1,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
