Application
Fig1: Western blot analysis of PRMT4 on different cell lysates using anti- PRMT4 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Hela Lane 3: NIH/3T3 Lane 4: HL-60 Lane 5: LNcap Lane 6: Jurkat Lane 7: PC-3 Lane 8: Cos7 Lane 9: PC-12Application
Fig2: ICC staining PRMT4 (green) and actin filaments (red) in Hela cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded breast cancer tissue using anti-PRMT4 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissue using anti-PRMT4 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of Lovo cells with PRMT4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Product Name | CARM1 [B10-H2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | carm1 antibody CARM1_HUMAN antibody Coactivator associated arginine methyltransferase 1 antibody Coactivator-associated arginine methyltransferase 1 antibody Histone arginine methyltransferase CARM 1 antibody Histone arginine methyltransferase CARM1 antibody Histone-arginine methyltransferase CARM1 antibody PRMT 4 antibody PRMT4 antibody Protein arginine methyltransferase antibody Protein arginine N methyltransferase 4 antibody Protein arginine N-methyltransferase 4 antibody |
| Product description | CARM1 (coactivator-associated arginine methyltransferase 1), also known as protein arginine N-methyltransferase 4 (PRMT4), is a 585 amino acid nuclear and cytoplasmic protein belonging to the protein arginine N-methyltransferase family. As a protein arginine N-methyltransferase, CARM1 is capable of catalyzing the transfer of methyl groups from S-adenosylmethionine to the guanidino group nitrogen atoms of arginine residues in certain proteins involved in mRNA stability, DNA packaging and transcriptional regulation. The methyltransferase activity of CARM1 has been found to be negatively regulated through phosphorylation at a conserved serine residue. CARM1 acts as a positive regulator for multiple transcription factors and functions as a secondary coactivator through its association with p160 coactivators. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:100-1:500 FC:1:100-1:200 |
|
| Species Reactivity | HumanMonkeyRatMouse |
| Concentration | 2mg/ml |
| Alternative Names | carm1 antibody CARM1_HUMAN antibody Coactivator associated arginine methyltransferase 1 antibody Coactivator-associated arginine methyltransferase 1 antibody Histone arginine methyltransferase CARM 1 antibody Histone arginine methyltransferase CARM1 antibody Histone-arginine methyltransferase CARM1 antibody PRMT 4 antibody PRMT4 antibody Protein arginine methyltransferase antibody Protein arginine N methyltransferase 4 antibody Protein arginine N-methyltransferase 4 antibody |
|---|---|
| Molecular Weight(MW) | 65 kDa |
| Cellular Localization | Cytoplasm. Nucleus. Nucleoplasm. |
Application
Fig1: Western blot analysis of PRMT4 on different cell lysates using anti- PRMT4 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Hela Lane 3: NIH/3T3 Lane 4: HL-60 Lane 5: LNcap Lane 6: Jurkat Lane 7: PC-3 Lane 8: Cos7 Lane 9: PC-12
Application
Fig2: ICC staining PRMT4 (green) and actin filaments (red) in Hela cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded breast cancer tissue using anti-PRMT4 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissue using anti-PRMT4 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of Lovo cells with PRMT4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Positive Control | MCF-7, Hela, NIH/3T3, HL-60, LNcap, Jurkat, PC-3, Cos7, PC-12, breast cancer tissue, ovarian cancer tissue, Lovo. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:100-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
