Application
Fig1: Western blot analysis of BLK on HEK293 (1) and BLK-hIgGFc transfected HEK293 (2) cell lysate using anti-BLK antibody at 1/1,000 dilution.Application
Fig2: ICC staining BLK (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Flow cytometric analysis of HL-60 cells with BLK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Product Name | BLK [A5-E6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | B lymphocyte kinase antibody B lymphoid tyrosine kinase antibody Blk antibody BLK nonreceptor tyrosine kinase antibody BLK_HUMAN antibody EC 2.7.10.2 antibody MGC 10442 antibody MGC10442 antibody MODY 11 antibody MODY11 antibody P55 BLK antibody p55-BLK antibody Tyrosine kinase B lymphocyte specific antibody Tyrosine protein kinase antibody Tyrosine-protein kinase BLK antibody |
| Product description | Src is the human homolog of the v-Src gene of the Rous sarcoma virus, also known as avian sarcoma virus or ASV. Src was the first proto-oncogenic non-receptor tyrosine kinase characterized in human. Based on common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Src family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility and adhesion. Src-family kinases contain an amino terminal cell membrane anchor followed by an SH3 domain and an SH2 domain involved in modular association and activation, respectively. Src family kinases are normally maintained in an inactive state and can be activated transiently during cellular events such as mitosis. Different subcellular localizations of Src-family kinases may be important for the regulation of specific cellular processes such as mitogenesis, cyto-skeletal organization and membrane trafficking. The human B lymphocyte kinase gene maps to chromosome 8p23.1 and encodes a 505 amino acid protein, known as Blk. Blk is expressed exclusively by B lymphocytes. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:1,000 ICC:1:100-1:500 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | B lymphocyte kinase antibody B lymphoid tyrosine kinase antibody Blk antibody BLK nonreceptor tyrosine kinase antibody BLK_HUMAN antibody EC 2.7.10.2 antibody MGC 10442 antibody MGC10442 antibody MODY 11 antibody MODY11 antibody P55 BLK antibody p55-BLK antibody Tyrosine kinase B lymphocyte specific antibody Tyrosine protein kinase antibody Tyrosine-protein kinase BLK antibody |
|---|---|
| Molecular Weight(MW) | 58 kDa |
| Cellular Localization | Membrane. Cytosol. |
| SwissProt ID | P51451 |
|---|
Application
Fig1: Western blot analysis of BLK on HEK293 (1) and BLK-hIgGFc transfected HEK293 (2) cell lysate using anti-BLK antibody at 1/1,000 dilution.
Application
Fig2: ICC staining BLK (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Flow cytometric analysis of HL-60 cells with BLK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Positive Control | BLK-hIgGFc transfected HEK293 cell lysate, Hela, HL-60. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:100-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
