Application
Fig1: Western blot analysis of SETDB1 on different cell lysates using anti-SETDB1 antibody at 1/1,000 dilution. Positive control： Lane1: MCF-7 Lane2: T47D Lane3: HEK293 Lane4: JURKAT Lane5: NIH/3T3 Lane6: F9 Lane7: RAW246.7 Lane8: Cos7Application
Fig2: ICC staining SETDB1 (green) and actin filaments (red) in LOVO cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | SETDB1 [A12-H6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AU022152 antibody EC 2.1.1.43 antibody ERG-associated protein with SET domain antibody ESET antibody H3 K9 HMTase 4 antibody H3-K9-HMTase 4 antibody H3-K9-HMTase4 antibody Histone H3 K9 methyltransferase 4 antibody Histone H3-K9 methyltransferase 4 antibody Histone-lysine N-methyltransferase SETDB1 antibody KG1T antibody KIAA0067 antibody KMT1E antibody Lysine N-methyltransferase 1E antibody MGC90670 antibody mKIAA0067 antibody SET domain bifurcated 1 antibody Set domain protein, bifurcated, 1 antibody SETB1_HUMAN antibody Setdb1 antibody |
| Product description | ERG-associated protein with SET domain (ESET), also designated Histone H3-K9 methyltransferase 4 or SET domain bifurcated 1, is a nuclear protein belonging to the histone-lysine methyltransferase family and to the Suvar3-9 subfamily. It is a highly conserved protein of 150 amino acids that has been implicated in chromatin structure modulation. ESET is excluded from cell nucleoli and areas of condensed chromatin and can associate with the nonpericentromeric regions of chromatin. The gene encoding for this protein, SETDB1, maps to chromosome 1q21. ESET is a histone methyltransferase, methylating Lys-9 of histone H3 and mutations within the SETDB1 gene abolishes its methyltransferase activity. This methylation acts as a tag for epigenetic transcriptional repression by rounding up HP1 proteins to methylated histones. ESET is widely expressed with highest levels found in testis. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICC |
| WB:1:500-1:2,000 ICC:1:200-1:1,000 |
|
| Species Reactivity | HumanMouseMonkey |
| Concentration | 2mg/ml |
| Alternative Names | AU022152 antibody
EC 2.1.1.43 antibody
ERG-associated protein with SET domain antibody
ESET antibody
H3 K9 HMTase 4 antibody
H3-K9-HMTase 4 antibody
H3-K9-HMTase4 antibody
Histone H3 K9 methyltransferase 4 antibody
Histone H3-K9 methyltransferase 4 antibody
Histone-lysine N-methyltransferase SETDB1 antibody
KG1T antibody
KIAA0067 antibody
KMT1E antibody
Lysine N-methyltransferase 1E antibody
MGC90670 antibody
mKIAA0067 antibody
SET domain bifurcated 1 antibody
Set domain protein bifurcated 1 antibody SETB1_HUMAN antibody Setdb1 antibody |
|---|---|
| Molecular Weight(MW) | 180 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of SETDB1 on different cell lysates using anti-SETDB1 antibody at 1/1,000 dilution. Positive control： Lane1: MCF-7 Lane2: T47D Lane3: HEK293 Lane4: JURKAT Lane5: NIH/3T3 Lane6: F9 Lane7: RAW246.7 Lane8: Cos7
Application
Fig2: ICC staining SETDB1 (green) and actin filaments (red) in LOVO cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | MCF-7, T47D, HEK293, JURKAT, NIH/3T3, F9, RAW246.7, Cos7, LOVO. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:200-1:1,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
