Application
Fig1: Western blot analysis of CRTC2 on Hela (1) and HEK293 (2) cell lysate using anti-CRTC2 antibody at 1/1,000 dilution.Application
Fig2: ICC staining CRTC2 (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human ovary tumour tissue using anti-CRTC2 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-CRTC2 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of Hela cells with CRTC2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Product Name | CRTC2 [D2-B10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CREB regulated transcription coactivator 2 antibody CREB-regulated transcription coactivator 2 antibody CRTC2 antibody CRTC2_HUMAN antibody RP11-422P24.6 antibody TORC-2 antibody torc2 antibody Transducer of CREB protein 2 antibody Transducer of regulated cAMP response element-binding protein antibody Transducer of regulated cAMP response element-binding protein (CREB) 2 antibody Transducer of regulated cAMP response element-binding protein 2 antibody Transducer of regulated CREB protein 2 antibody |
| Product description | CRTC2 also named TORC2. The TORC (transducer of regulated cAMP response element-binding) proteins, TORC1 and TORC2, are potent CREB coactivators that are exported from the nucleus in a CRM1-dependent manner. The translocation of TORC proteins is a conserved step in the activation of CRE-mediated gene expression induced by cAMP. TORC1 and TORC2 operate via phosphorylation-dependent interactions. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | HumanMonkey |
| Concentration | 2mg/ml |
| Alternative Names | CREB regulated transcription coactivator 2 antibody CREB-regulated transcription coactivator 2 antibody CRTC2 antibody CRTC2_HUMAN antibody RP11-422P24.6 antibody TORC-2 antibody torc2 antibody Transducer of CREB protein 2 antibody Transducer of regulated cAMP response element-binding protein antibody Transducer of regulated cAMP response element-binding protein (CREB) 2 antibody Transducer of regulated cAMP response element-binding protein 2 antibody Transducer of regulated CREB protein 2 antibody |
|---|---|
| Molecular Weight(MW) | 80 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
| SwissProt ID | Q53ET0 |
|---|
Application
Fig1: Western blot analysis of CRTC2 on Hela (1) and HEK293 (2) cell lysate using anti-CRTC2 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining CRTC2 (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human ovary tumour tissue using anti-CRTC2 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-CRTC2 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of Hela cells with CRTC2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Positive Control | Hela, HEK293, human ovary tumour tissue, human lung cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
