Application
Fig1: Western blot analysis of CSK on different cell lysates using anti-CSK antibody at 1/1,000 dilution. Positive control： Lane1: NIH/3T3 Lane2: Hela Lane3: COS7 Lane4: Jurkat Lane5: Raw246.7 Lane6: A549 Lane7: HL-60 Lane8: PC-12Application
Fig2: ICC staining CSK (green) and actin filaments (red) in U251 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Flow cytometric analysis of HL-60 cells with CSK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Product Name | CSK [F1-F3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C SRC antibody C SRC kinase antibody C src Tyrosine Kinase antibody C-SRC kinase antibody c-src tyrosine kinase antibody Csk A antibody CSK antibody CSK_HUMAN antibody CYTOPLASMIC TYROSINE KINASE antibody EC 2.7.10.2 antibody MGC112926 antibody MGC117393 antibody MGC154049 antibody P60 Src antibody Protein tyrosine kinase CYL antibody Protein-tyrosine kinase CYL antibody Proto oncogene tyrosine protein kinase antibody Tyrosine protein kinase CSK antibody Tyrosine-protein kinase CSK antibody zgc:154049 antibody |
| Product description | All members of the Src gene family of tyrosine kinases are characterized by a carboxy terminal domain tyrosine which is highly phosphorylated in the inactive form of the enzyme and phosphorylated to a much lesser extent when the enzyme is active. In the case of Src p60, Y527 is this tyrosine; however, a mutant form of c-Src in which Y527 is replaced by phenylalanine is transforming and displays 5- to 10-fold elevated kinase activity compared to its normal counterpart. Csk has been identified as a Src-related tyrosine kinase having both SH2 and SH3 domains and a catalytic domain but lacking sequences amino terminal to the SH3 domain as well as carboxy terminal regulatory sequences. Csk phosphorylates Src on Y527 and also downregulates Lyn, Fyn and Lck by tyrosine phosphorylation of carboxy terminal regulatory sites. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:1,000 ICC:1:100-1:500 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouseRatMonkey |
| Concentration | 2mg/ml |
| Alternative Names | C SRC antibody C SRC kinase antibody C src Tyrosine Kinase antibody C-SRC kinase antibody c-src tyrosine kinase antibody Csk A antibody CSK antibody CSK_HUMAN antibody CYTOPLASMIC TYROSINE KINASE antibody EC 2.7.10.2 antibody MGC112926 antibody MGC117393 antibody MGC154049 antibody P60 Src antibody Protein tyrosine kinase CYL antibody Protein-tyrosine kinase CYL antibody Proto oncogene tyrosine protein kinase antibody Tyrosine protein kinase CSK antibody Tyrosine-protein kinase CSK antibody zgc:154049 antibody |
|---|---|
| Molecular Weight(MW) | 51 kDa |
| Cellular Localization | Cytoplasm. Cell membrane. |
Application
Fig1: Western blot analysis of CSK on different cell lysates using anti-CSK antibody at 1/1,000 dilution. Positive control： Lane1: NIH/3T3 Lane2: Hela Lane3: COS7 Lane4: Jurkat Lane5: Raw246.7 Lane6: A549 Lane7: HL-60 Lane8: PC-12
Application
Fig2: ICC staining CSK (green) and actin filaments (red) in U251 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Flow cytometric analysis of HL-60 cells with CSK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Positive Control | NIH/3T3, Hela, COS7, Jurkat, Raw246.7, A549, HL-60, PC-12, U251. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:100-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
