Application
Fig1: Western blot analysis of CBL on human CBL recombinant protein using anti-CBL antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of CBL on different cell lysates using anti-CBL antibody at 1/1,000 dilution. Positive control: Lane 1: RAJI Lane 2: RAW264.7 Lane 3: K562 Lane 4: SKBR-3 Lane 5: 3T3-L1 Lane 6: THP-1 Lane 7: PC-12Application
Fig3: ICC staining CBL (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissue using anti-CBL antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded bladder cancer tissue using anti-CBL antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of MCF-7 cells with CBL antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | C-CBL [C1-B12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 4732447J05Rik antibody C CBL antibody Cas Br M (murine) ecotropic retroviral transforming sequence antibody Casitas B lineage lymphoma proto oncogene antibody Casitas B-lineage lymphoma proto-oncogene antibody CBL 2 antibody cbl antibody CBL_HUMAN antibody CBL2 antibody E3 ubiquitin protein ligase CBL antibody E3 ubiquitin-protein ligase CBL antibody Oncogene CBL2 antibody Proto oncogene c CBL antibody Proto-oncogene c-CBL antibody RGD1561386 antibody RING finger protein 55 antibody RNF55 antibody Signal transduction protein CBL antibody |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | 4732447J05Rik antibody C CBL antibody Cas Br M (murine) ecotropic retroviral transforming sequence antibody Casitas B lineage lymphoma proto oncogene antibody Casitas B-lineage lymphoma proto-oncogene antibody CBL 2 antibody cbl antibody CBL_HUMAN antibody CBL2 antibody E3 ubiquitin protein ligase CBL antibody E3 ubiquitin-protein ligase CBL antibody Oncogene CBL2 antibody Proto oncogene c CBL antibody Proto-oncogene c-CBL antibody RGD1561386 antibody RING finger protein 55 antibody RNF55 antibody Signal transduction protein CBL antibody |
|---|---|
| Molecular Weight(MW) | 120 kDa |
| Cellular Localization | Cell membrane, Cytoplasm. |
Application
Fig1: Western blot analysis of CBL on human CBL recombinant protein using anti-CBL antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CBL on different cell lysates using anti-CBL antibody at 1/1,000 dilution. Positive control: Lane 1: RAJI Lane 2: RAW264.7 Lane 3: K562 Lane 4: SKBR-3 Lane 5: 3T3-L1 Lane 6: THP-1 Lane 7: PC-12
Application
Fig3: ICC staining CBL (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissue using anti-CBL antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded bladder cancer tissue using anti-CBL antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of MCF-7 cells with CBL antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human C-CBL recombinant protein, RAJI, RAW264.7, K562, SKBR-3, 3T3-L1, THP-1, PC-12, Hela, ovarian cancer tissue, bladder cancer tissue, MCF-7. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
