Application
Fig1: Western blot analysis of CRK on human CRK recombinant protein using anti-CRK antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of CRK on HEK293 (1) and CRK-hIgGFc transfected HEK293 (2) cell lysate using anti-CRK antibody at 1/1,000 dilution.Application
Fig3: ICC staining CRK (green) and actin filaments (red) in 3T3-L1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-CRK antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-CRK antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of MCF-7 cells with CRK antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | CRK [C1-G11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Adapter molecule crk antibody Avian sarcoma virus CT10 (v crk) oncogene homolog antibody CRK antibody CRK isoform 2 antibody CRK isoform II antibody CRK_HUMAN antibody CRKII antibody FLJ38130 antibody OTTHUMP00000115366 antibody OTTHUMP00000198330 antibody p38 antibody Proto oncogene C crk antibody Proto-oncogene C-crk antibody v crk avian sarcoma virus CT10 oncogene homolog antibody v crk sarcoma virus CT10 oncogene homolog antibody v crk sarcoma virus CT10 oncogene homolog (avian) antibody |
| Product description | The Crk family of adapter proteins including Crk-II, Crk-I, and Crk-L consist mostly of SH2 and SH3 domains. Crk I and Crk II are encoded by the same gene, which undergoes alternative splicing to yield these two proteins, but differ in their biological activities . Crk-II has less transforming activity than Crk-I, although both Crk-I and Crk-II bind to many tyrosine-phosphorylated proteins that bind to grb2. In addition, Crk-II becomes rapidly tyrosine-phosphorylated in response to stimulation with insulin-like growth factor I (IGF-I) and might be involved in the IGF-I receptor signalling pathway. The gene encoding Crk I and II maps to human chromosome 17p13, a region which demonstrates frequent deletion or loss of heterozygosity in a wide range of human cancers. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Adapter molecule crk antibody Avian sarcoma virus CT10 (v crk) oncogene homolog antibody CRK antibody CRK isoform 2 antibody CRK isoform II antibody CRK_HUMAN antibody CRKII antibody FLJ38130 antibody OTTHUMP00000115366 antibody OTTHUMP00000198330 antibody p38 antibody Proto oncogene C crk antibody Proto-oncogene C-crk antibody v crk avian sarcoma virus CT10 oncogene homolog antibody v crk sarcoma virus CT10 oncogene homolog antibody v crk sarcoma virus CT10 oncogene homolog (avian) antibody |
|---|---|
| Molecular Weight(MW) | 38 kDa |
| Cellular Localization | Cytooplasm. Cell membrane. |
| SwissProt ID | P46108 |
|---|
Application
Fig1: Western blot analysis of CRK on human CRK recombinant protein using anti-CRK antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CRK on HEK293 (1) and CRK-hIgGFc transfected HEK293 (2) cell lysate using anti-CRK antibody at 1/1,000 dilution.
Application
Fig3: ICC staining CRK (green) and actin filaments (red) in 3T3-L1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-CRK antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-CRK antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of MCF-7 cells with CRK antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human CRK recombinant protein, CRK-hIgGFc transfected HEK293 cell lysate, human rectum cancer tissue, human bladder cancer tissue, 3T3-L1, MCF-7. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
