Application
Fig1: Western blot analysis of FYN on human FYN recombinant protein using anti-FYN antibody at 1/1,000 dilution.Application
Fig2: ICC staining FYN (green) and actin filaments (red) in U251 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-FYN antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-FYN antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of Hela cells with FYN antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Product Name | FYN [D2-A10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C syn protooncogene antibody Fyn antibody FYN oncogene related to SRC FGR YES antibody FYN_HUMAN antibody OKT3 induced calcium influx regulator antibody P59 FYN antibody p59-Fyn antibody Protein tyrosine kinase fyn antibody Proto oncogene tyrosine protein kinase fyn antibody Proto-oncogene c-Fyn antibody Proto-oncogene Syn antibody Protooncogene Syn antibody SLK antibody Src like kinase antibody Src yes related novel gene antibody Src-like kinase antibody Src/yes related novel antibody SYN antibody Tyrosine kinase p59fyn T antibody Tyrosine kinase p59fyn(T) antibody Tyrosine-protein kinase Fyn antibody |
| Product description | By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, and adhesion. Src-family kinases contain an amino terminal cell membrane anchor followed by an SH3 domain and an SH2 domain involved in modular association and activation, respectively. Different subcellular localizations of Src-family kinases may be important for the regulation of specific cellular processes such as mitogenesis, cytoskeletal organization, and membrane trafficking. Fyn and Lck kinases play a key role in T-cell antigen receptor (TCR) signaling. The human Fyn gene maps to chromosome 6q21 and encodes a 537 amino acid protein. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | C syn protooncogene antibody Fyn antibody FYN oncogene related to SRC FGR YES antibody FYN_HUMAN antibody OKT3 induced calcium influx regulator antibody P59 FYN antibody p59-Fyn antibody Protein tyrosine kinase fyn antibody Proto oncogene tyrosine protein kinase fyn antibody Proto-oncogene c-Fyn antibody Proto-oncogene Syn antibody Protooncogene Syn antibody SLK antibody Src like kinase antibody Src yes related novel gene antibody Src-like kinase antibody Src/yes related novel antibody SYN antibody Tyrosine kinase p59fyn T antibody Tyrosine kinase p59fyn(T) antibody Tyrosine-protein kinase Fyn antibody |
|---|---|
| Molecular Weight(MW) | 61 kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P06241 |
|---|
Application
Fig1: Western blot analysis of FYN on human FYN recombinant protein using anti-FYN antibody at 1/1,000 dilution.
Application
Fig2: ICC staining FYN (green) and actin filaments (red) in U251 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-FYN antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-FYN antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of Hela cells with FYN antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Positive Control | Human FYN recombinant protein, U251, breast cancer tissue, brain tissue, Hela. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
