Application
Fig1: Western blot analysis of EIF4B on human EIF4B recombinant protein using anti-EIF4B antibody at 1/1,000 dilution.Application
ApplicationApplication
Fig3: ICC staining EIF4B (green) and actin filaments (red) in NIH-3T3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded colon cancer tissue using anti-EIF4B antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of Hela cells with EIF4B antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | EIF4B [D7-F5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 2310046H11Rik antibody AL024095 antibody C85189 antibody EIF 4B antibody eIF-4B antibody EIF4B antibody Eukaryotic initiation factor 4B antibody Eukaryotic translation initiation factor 4B antibody IF4B_HUMAN antibody PRO1843 antibody |
| Product description | The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. These interactions are facilitated, in part, by the eukaryotic initiation factor 4 family (eIF4) of proteins that are involved in the early initiation of protein synthesis. eIF4B (eukaryotic translation initiation factor 4B) is a 611 amino acid protein that contains one RNA recognition motif and belongs to the eIF4 protein family. Required for proper mRNA/ribosome binding, eIF4B associates with other eIF4 proteins, such as eIF4A, and promotes the ATP-dependent unwinding activity of select eukaryotic initiation factors. The gene encoding eIF4B maps to human chromosome 12, which encodes over 1,100 genes and comprises approximately 4.5% of the human genome. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:100-1:500 IHC:1:100-1:500 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | 2310046H11Rik antibody AL024095 antibody C85189 antibody EIF 4B antibody eIF-4B antibody EIF4B antibody Eukaryotic initiation factor 4B antibody Eukaryotic translation initiation factor 4B antibody IF4B_HUMAN antibody PRO1843 antibody |
|---|---|
| Molecular Weight(MW) | 80 kDa |
| Cellular Localization | Cytosol. |
Application
Fig1: Western blot analysis of EIF4B on human EIF4B recombinant protein using anti-EIF4B antibody at 1/1,000 dilution.
Application
Application
Application
Fig3: ICC staining EIF4B (green) and actin filaments (red) in NIH-3T3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded colon cancer tissue using anti-EIF4B antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of Hela cells with EIF4B antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human EIF4B recombinant protein, A431, A549, HepG2, HEK293, HeLa, Jurkat, K562, NIH-3T3, MCF-7, colon cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:100-1:500 IHC:1:100-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
