Application
Fig1: Western blot analysis of BLNK on human BLNK recombinant protein using anti-BLNK antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of BLNK on NIH/3T3 (1) and BCBL-1 (2) cell lysate using anti-BLNK antibody at 1/1,000 dilution.Application
Fig3: ICC staining BLNK (green) and actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-BLNK antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of NIH/3T3 cells with BLNK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Product Name | BLNK [G1-F9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AGM4 antibody B cell adapter containing SH2 domain protein antibody B cell adapter containing Src homology 2 domain protein antibody B cell adaptor containing SH2 domain antibody B cell linker antibody B cell linker protein antibody B cell-specific adaptor protein antibody B-cell activation antibody B-cell adapter containing a SH2 domain protein antibody B-cell adapter containing a Src homology 2 domain protein antibody B-cell linker protein antibody BASH antibody Bca antibody BLNK antibody BLNK s antibody BLNK_HUMAN antibody Cytoplasmic adapter protein antibody Ly 57 antibody Ly57 antibody Lymphocyte antigen 57 antibody Lyw 57 antibody Lyw57 antibody MGC111051 antibody SH2 domain-containing leukocyte protein, 65-KD antibody SLP 65 antibody SLP-65 antibody SLP65 antibody Src homology [SH2] domain-containing leukocyte protein of 65 kD antibody Src homology 2 domain containing leukocyte protein of 65 kDa antibody Src homology 2 domain-containing leukocyte protein of 65 kDa antibody |
| Product description | Cross-linking of the B cell receptor (BCR) activates a variety of signaling pathways involved in processes such as cell proliferation and apoptosis. Intracellular protein tyrosine kinases such as Syk and Lyn have been implicated in this BCR signal transduction and are thought to play an important role in B cell development. BLNK (for B cell linker protein) is a central linker protein in B cells which has been shown to associate with the effector proteins GRB2, Vav, Nck and PLC γ following activation of the B cell receptor. The two forms of BLNK, pp68 and pp70, arise from alternate splicing of the human BLNK gene transcript. BLNK is phosphorylated by the Syk tyrosine kinase, which in turn permits activation of downstream effector proteins including GRB2 and PLC γ. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:100-1:200 IHC:1:100-1:500 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | AGM4 antibody
B cell adapter containing SH2 domain protein antibody
B cell adapter containing Src homology 2 domain protein antibody
B cell adaptor containing SH2 domain antibody
B cell linker antibody
B cell linker protein antibody
B cell-specific adaptor protein antibody
B-cell activation antibody
B-cell adapter containing a SH2 domain protein antibody
B-cell adapter containing a Src homology 2 domain protein antibody
B-cell linker protein antibody
BASH antibody
Bca antibody
BLNK antibody
BLNK s antibody
BLNK_HUMAN antibody
Cytoplasmic adapter protein antibody
Ly 57 antibody
Ly57 antibody
Lymphocyte antigen 57 antibody
Lyw 57 antibody
Lyw57 antibody
MGC111051 antibody
SH2 domain-containing leukocyte protein 65-KD antibody SLP 65 antibody SLP-65 antibody SLP65 antibody Src homology [SH2] domain-containing leukocyte protein of 65 kD antibody Src homology 2 domain containing leukocyte protein of 65 kDa antibody Src homology 2 domain-containing leukocyte protein of 65 kDa antibody |
|---|---|
| Molecular Weight(MW) | 70 kDa |
| Cellular Localization | Cytosplasm. Cell membrane. |
Application
Fig1: Western blot analysis of BLNK on human BLNK recombinant protein using anti-BLNK antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of BLNK on NIH/3T3 (1) and BCBL-1 (2) cell lysate using anti-BLNK antibody at 1/1,000 dilution.
Application
Fig3: ICC staining BLNK (green) and actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-BLNK antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of NIH/3T3 cells with BLNK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Positive Control | Human BLNK recombinant protein, NIH/3T3, BCBL-1, HepG2, human cervical cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:100-1:200 IHC:1:100-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
