Application
Fig1: Western blot analysis of KLHL1 on human KLHL1 recombinant protein using anti-KLHL1 antibody at 1/1,000 dilution.Application
Fig2: ICC staining KLHL1 (green) and actin filaments (red) in NIH/3T3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | KLHL1 [B3-A8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ30047 antibody Kelch (Drosophila) like 1 antibody Kelch like 1 (Drosophila) antibody Kelch like 1 antibody Kelch like protein 1 antibody kelch-like family member 1 antibody Kelch-like protein 1 antibody KIAA1490 antibody KLHL1 antibody KLHL1_HUMAN antibody Mayven related protein 2 antibody MRP2 antibody OTTHUMP00000018492 antibody OTTHUMP00000040834 antibody |
| Product description | KLHL1 (kelch-like protein 1) is a 748 amino acid protein that is the homolog of the Drosophila kelch protein. Localized to the cytoskeleton, KLHL1 contains six kelch repeats and one BTB (POZ) domain. The BTB (Broad-Complex, Tramtrack and Bric a brac) domain, also known as the POZ (Poxvirus and Zinc finger) domain, is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. KLHL1 is highly expressed in brain where it acts as an actin-organization protein, possibly playing a role in the modulation of neurite outgrowth. KLHL1 expression has been shown to be downregulated by spinocerebellar ataxia type 8 (SCA8) protein, which then leads to SCA8 neuropathogenesis, a disease that is characterized by limb and truncal ataxia, ataxic dysarthria and horizontal nystagmus. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICC |
| WB:1:500-1:1,000 ICC:1:100-1:500 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | FLJ30047 antibody Kelch (Drosophila) like 1 antibody Kelch like 1 (Drosophila) antibody Kelch like 1 antibody Kelch like protein 1 antibody kelch-like family member 1 antibody Kelch-like protein 1 antibody KIAA1490 antibody KLHL1 antibody KLHL1_HUMAN antibody Mayven related protein 2 antibody MRP2 antibody OTTHUMP00000018492 antibody OTTHUMP00000040834 antibody |
|---|---|
| Molecular Weight(MW) | 82 kDa |
| Cellular Localization | Cytoplasm. Cytoskeleton. |
Application
Fig1: Western blot analysis of KLHL1 on human KLHL1 recombinant protein using anti-KLHL1 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining KLHL1 (green) and actin filaments (red) in NIH/3T3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | Human KLHL1 recombinant protein, NIH/3T3. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:100-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
