Application
Fig1: Western blot analysis of ApoB on human ApoB recombinant protein using anti-ApoB antibody at 1/1,000 dilution.Application
Fig2: ICC staining ApoB (green) and actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Flow cytometric analysis of PBMC cells with ApoB antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Product Name | ApoB [B1-G6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | apo b-100 antibody APOB antibody apoB-48R antibody APOBR antibody APOBR_HUMAN antibody Apolipoprotein B receptor antibody Apolipoprotein B-100 receptor antibody Apolipoprotein B-48 receptor antibody Apolipoprotein B48 receptor antibody |
| Product description | Post-transcriptional editing of apolipoprotein B (apoB) mRNA is regulated by APOBEC1 (also designated human (or rat) small intestinal apolipoprotein B mRNA editing protein, HEPR, or REPR) in hepatic cells to achieve a steady state proportion of edited and unedited RNA molecules. Two forms of apoB are known to circulate in the plasma of mammals. apoB-100 is a protein primarily synthesized in the liver as a structural component of very-low-density lipoprotein particles. A truncated form of apoB-100, apoB-48, is synthesized in the small intestine and contains the amino-terminal 2,152 amino acids of the larger protein. This organ-specific partitioning of apoB production is the result of RNA editing of a common apoB gene. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | apo b-100 antibody APOB antibody apoB-48R antibody APOBR antibody APOBR_HUMAN antibody Apolipoprotein B receptor antibody Apolipoprotein B-100 receptor antibody Apolipoprotein B-48 receptor antibody Apolipoprotein B48 receptor antibody |
|---|---|
| Molecular Weight(MW) | 516 kDa |
| Cellular Localization | Cytoplasm. Extracellular region. Secreted. |
| SwissProt ID | P04114 |
|---|
Application
Fig1: Western blot analysis of ApoB on human ApoB recombinant protein using anti-ApoB antibody at 1/1,000 dilution.
Application
Fig2: ICC staining ApoB (green) and actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Flow cytometric analysis of PBMC cells with ApoB antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Positive Control | Human ApoB recombinant protein, HepG2, PBMC. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
