Application
Fig1: Western blot analysis of CEBP alpha on different cell lysates using anti-CEBP alpha antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: k562 Lane 3: HepG2Application
Fig2: ICC staining CEBP alpha (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human rectum tissue tissue using anti-CEBP alpha antibody. Counter stained with hematoxylin.Application
Fig4: Flow cytometric analysis of MCF-7 cells with CEBP alpha antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | CEBPA [E8-B7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Apoptotic cysteine protease antibody Apoptotic protease Mch 5 antibody C/EBP alpha antibody C/ebpalpha antibody CAP4 antibody Caspase 8 precursor antibody CBF-A antibody CCAAT Enhancer Binding Protein alpha antibody CCAAT/enhancer binding protein (C/EBP), alpha antibody CCAAT/enhancer-binding protein alpha antibody CEBP antibody CEBP A antibody CEBP alpha antibody Cebpa antibody CEBPA_HUMAN antibody FADD homologous ICE/CED 3 like protease antibody FADD like ICE antibody FLICE antibody ICE like apoptotic protease 5 antibody ICE8 antibody MACH antibody MCH5 antibody MORT1 associated CED 3 homolog antibody |
| Product description | The transcription factor C/EBP α (CCAAT-enhancer binding protein) is a heat-stable, sequence-specific DNA-binding protein that binds avidly to several different cis-regulatory DNA sequences commonly associated with viral and cellular genes transcribed by RNA polymerase II. C/EBP α regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP α is a basic region/leucine zipper transcription factor selectively expressed during the differentiation of liver, adipose tissue, blood cells and the endocrine pancreas. C/EBP α uses a bipartite structural motif to bind DNA and appears to function exclusively in terminally differentiated, growth-arrested cells. In the liver, C/EBP α is a transactivator of several genes, which are regulated by growth hormone. Growth hormone enhances not only the levels of C/EBP α mRNA and protein, but also the DNA binding activity of C/EBP α. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500 ICC:1:100-1:500 IHC:1:100-1:500 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Apoptotic cysteine protease antibody
Apoptotic protease Mch 5 antibody
C/EBP alpha antibody
C/ebpalpha antibody
CAP4 antibody
Caspase 8 precursor antibody
CBF-A antibody
CCAAT Enhancer Binding Protein alpha antibody
CCAAT/enhancer binding protein (C/EBP) alpha antibody CCAAT/enhancer-binding protein alpha antibody CEBP antibody CEBP A antibody CEBP alpha antibody Cebpa antibody CEBPA_HUMAN antibody FADD homologous ICE/CED 3 like protease antibody FADD like ICE antibody FLICE antibody ICE like apoptotic protease 5 antibody ICE8 antibody MACH antibody MCH5 antibody MORT1 associated CED 3 homolog antibody |
|---|---|
| Molecular Weight(MW) | 42 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P49715 |
|---|
Application
Fig1: Western blot analysis of CEBP alpha on different cell lysates using anti-CEBP alpha antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: k562 Lane 3: HepG2
Application
Fig2: ICC staining CEBP alpha (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human rectum tissue tissue using anti-CEBP alpha antibody. Counter stained with hematoxylin.
Application
Fig4: Flow cytometric analysis of MCF-7 cells with CEBP alpha antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Application Notes | WB:1:500 ICC:1:100-1:500 IHC:1:100-1:500 FC:1:100-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
