Application
Fig1: Western blot analysis of Fucokinase on different cell lysates using anti- Fucokinase antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: Jurkat Lane 4: A431 Lane 5: HEK293 Lane 6: MCF-7 Lane 7: PC-12 Lane 8: Cos7 Lane 9:NIH/3T3Application
Fig2: Flow cytometric analysis of Hela cells with Fucokinase antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Product Name | FUK [E2-C3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The protein encoded by this gene belongs to the GHMP (galacto-, homoserine, mevalonate and phosphomevalonate) kinase family and catalyzes the phosphorylation of L-fucose to form beta-L-fucose 1-phosphate. This enzyme catalyzes the first step in the utilization of free L-fucose in glycoprotein and glycolipid synthesis. L-fucose may be important in mediating a number of cell-cell interactions such as blood group antigen recognition, inflammation, and metastatis. While several transcript variants may exist for this gene, the full-length nature of only one has been described to date. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:1,000 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouseRatMonkey |
| Concentration | 2mg/ml |
| Molecular Weight(MW) | 117 kDa, additional band 42kDa |
|---|---|
| Cellular Localization | Cytosol. |
Application
Fig1: Western blot analysis of Fucokinase on different cell lysates using anti- Fucokinase antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: Jurkat Lane 4: A431 Lane 5: HEK293 Lane 6: MCF-7 Lane 7: PC-12 Lane 8: Cos7 Lane 9:NIH/3T3
Application
Fig2: Flow cytometric analysis of Hela cells with Fucokinase antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Positive Control | Hela, HepG2, Jurkat, A431, HEK293, MCF-7, PC-12, Cos7 , NIH/3T3. |
|---|---|
| Application Notes | WB:1:500-1:1,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
