Application
Fig1: Western blot analysis of Hexokinase 2 on different cell lysates using anti- Hexokinase 2 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: Hela Lane 3: HEK293Application
Fig2: Immunohistochemical analysis of paraffin-embedded human esophagus cancer tissue using anti- Hexokinase 2 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti- Hexokinase 2 antibody. Counter stained with hematoxylin.Application
Fig4: Flow cytometric analysis of K562 cells with Hexokinase 2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Product Name | HK2 [F1-D3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp686M1669 antibody Hexokinase 2 antibody Hexokinase 2 muscle antibody Hexokinase type II antibody Hexokinase-2 antibody HK 2 antibody HK II antibody HK2 antibody HKII antibody HxK 2 antibody HxK2 antibody HXK2_HUMAN antibody Muscle form hexokinase antibody |
| Product description | The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose-6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases. HXK I has been shown to be expressed in brain, kidney and heart tissues as well as in hepatoma cell lines. HXK II is involved in the uptake and utilization of glucose by adipose and skeletal tissues. Of the hexokinases, HXK III has the highest affinity for glucose. Glucokinase is expressed in pancreatic beta cells where it functions as a glucose sensor, determining the "set point" for insulin secretion. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:1,000 IHC:1:100-1:500 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | DKFZp686M1669 antibody Hexokinase 2 antibody Hexokinase 2 muscle antibody Hexokinase type II antibody Hexokinase-2 antibody HK 2 antibody HK II antibody HK2 antibody HKII antibody HxK 2 antibody HxK2 antibody HXK2_HUMAN antibody Muscle form hexokinase antibody |
|---|---|
| Molecular Weight(MW) | 102 kDa |
| Cellular Localization | Mitochondrion outer membrane. |
| SwissProt ID | P52789 |
|---|
Application
Fig1: Western blot analysis of Hexokinase 2 on different cell lysates using anti- Hexokinase 2 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: Hela Lane 3: HEK293
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human esophagus cancer tissue using anti- Hexokinase 2 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti- Hexokinase 2 antibody. Counter stained with hematoxylin.
Application
Fig4: Flow cytometric analysis of K562 cells with Hexokinase 2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Positive Control | Jurkat, Hela, HEK293, human esophagus cancer tissue, human lung cancer tissue, K562. |
|---|---|
| Application Notes | WB:1:500-1:1,000 IHC:1:100-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
