Application
Fig1: Western blot analysis of TWF1 on human TWF1 recombinant protein using anti-TWF1 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of TWF1 on HEK293 (1) and TWF1-hIgGFc transfected HEK293 (2) cell lysate using anti-TWF1 antibody at 1/1,000 dilution.Application
Fig3: ICC staining TWF1 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-TWF1 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-TWF1 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Hela cells with TWF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | TWF1 [A2-C11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A6 antibody A6 protein tyrosine kinase antibody MGC105817 antibody MGC23788 antibody MGC41876 antibody Protein A6 antibody Protein tyrosine kinase 9 antibody PTK9 antibody PTK9 protein tyrosine kinase 9 antibody TWF 1 antibody twf1 antibody TWF1_HUMAN antibody Twinfilin 1 antibody Twinfilin actin binding protein, homolog 1 antibody Twinfilin antibody Twinfilin, actin-binding protein, homolog 1 (Drosophila) antibody Twinfilin, Drosophila, homolog of, 1 antibody Twinfilin-1 antibody |
| Product description | Twinfilin is a highly conserved Actin monomer-binding protein that regulates cytoskeletal dynamics in organisms from yeast to mammals. Twinfilin is composed of two ADF-homology domains; it coordinates filament severing and monomer sequestering at sites of rapid Actin turnover, thus preventing assembly of the monomer into filaments. Twinfilin-1 is the mammalian homolog and is expressed in embryos and in most adult non-muscle cell types. Twinfilin-1 binds ADP-G-Actin and efficiently halts Actin filament assembly by inhibiting the nucleotide exchange on Actin monomers and directly interacting with the capping protein. Phosphatidylinositol (4,5)-bisphosphate inhibits the activity of Twinfilin-1, while two small GTPases, namely Rac1 and Cdc42, induce the redistribution of Twinfilin-1 to membrane ruffles and cell-cell contacts, respectively. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:50-1:100 IHC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | A6 antibody
A6 protein tyrosine kinase antibody
MGC105817 antibody
MGC23788 antibody
MGC41876 antibody
Protein A6 antibody
Protein tyrosine kinase 9 antibody
PTK9 antibody
PTK9 protein tyrosine kinase 9 antibody
TWF 1 antibody
twf1 antibody
TWF1_HUMAN antibody
Twinfilin 1 antibody
Twinfilin actin binding protein homolog 1 antibody Twinfilin antibody Twinfilin actin-binding protein homolog 1 (Drosophila) antibody Twinfilin Drosophila homolog of 1 antibody Twinfilin-1 antibody |
|---|---|
| Molecular Weight(MW) | 40 kDa |
| Cellular Localization | Cytoplasm. Cytoskeleton. |
| SwissProt ID | Q12792 |
|---|
Application
Fig1: Western blot analysis of TWF1 on human TWF1 recombinant protein using anti-TWF1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of TWF1 on HEK293 (1) and TWF1-hIgGFc transfected HEK293 (2) cell lysate using anti-TWF1 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining TWF1 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-TWF1 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-TWF1 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with TWF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human TWF1 recombinant protein, TWF1-hIgGFc transfected HEK293 cell lysate, Hela, human ovarian cancer tissue, human bladder cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:100 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
