Application
Fig1: Western blot analysis of INCENP on human INCENP recombinant protein using anti-INCENP antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of INCENP on HEK293 (1) and INCENP-hIgGFc transfected HEK293 (2) cell lysate using anti-INCENP antibody at 1/1,000 dilution.Application
Fig3: ICC staining INCENP (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4:Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-INCENP antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-INCENP antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Jurkat cells with INCENP antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | INCENP [G9-D2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | binds and activates aurora B and C in vivo and in vitro antibody Chromosomal passenger protein antibody INCE_HUMAN antibody INCENP antibody Inner centromere protein antibody Inner centromere protein antigens 135/155kDa antibody Inner centromere protein antigens 135kD 155kD antibody Inner centromere protein INCENP antibody |
| Product description | A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The centromere proteins CENP-A, CENP-B, CENP-C, CENP-E, CENP-F (also designated mitosin), CENP-H and INCENP are kinetochore proteins that are involved in mitotic events. The centromere proteins are expressed at different levels throughout the cell cycle and are involved in the formation of the centromere and the organization and function of the kinetochore. INCENP, which also is designated inner centromere protein, is a chromosomal passenger protein that is crucial for chromosome segregation. During mitosis it is also required for cytokinesis onset. This protein, which can form a homodimer or a heterodimer, binds directly to microtubules and interacts with AURKB, AURKC, CBX3 and β Tubulin. This nuclear protein localizes to the mitotic spindle, methaphase chromosomes and during anaphase, to the equatorial cortex. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | binds and activates aurora B and C in vivo and in vitro antibody Chromosomal passenger protein antibody INCE_HUMAN antibody INCENP antibody Inner centromere protein antibody Inner centromere protein antigens 135/155kDa antibody Inner centromere protein antigens 135kD 155kD antibody Inner centromere protein INCENP antibody |
|---|---|
| Molecular Weight(MW) | 105 kDa |
| Cellular Localization | Cytoskeleton. Nucleus. |
| SwissProt ID | Q9NQS7 |
|---|
Application
Fig1: Western blot analysis of INCENP on human INCENP recombinant protein using anti-INCENP antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of INCENP on HEK293 (1) and INCENP-hIgGFc transfected HEK293 (2) cell lysate using anti-INCENP antibody at 1/1,000 dilution.
Application
Fig3: ICC staining INCENP (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-INCENP antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-INCENP antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Jurkat cells with INCENP antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human INCENP recombinant protein, INCENP-hIgGFc transfected HEK293 cell lysate, human cervical cancer tissue, human rectum cancer tissue, HepG2, Jurkat. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
